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Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica
TLDR
Differences in these clusters reflect important structural variations in the outer core oligosaccharides and provide a basis for ascribing functions to the genes in these model clusters, whereas highly conserved regions within these clusters suggest a critical and unalterable function for the inner region of the core. Expand
Expression of the multidrug resistance operon mexA-mexB-oprM in Pseudomonas aeruginosa: mexR encodes a regulator of operon expression
TLDR
The region upstream of the multiple antibiotic resistance efflux operon mexA-mexB-oprM in Pseudomonas aeruginosa was sequenced, and a gene, mexR, was identified, suggesting that MexR possesses both repressor and activator function in vivo, the activator form being favored in nalB multidrug-resistant strains. Expand
Distribution of Core Oligosaccharide Types in Lipopolysaccharides from Escherichia coli
TLDR
Unique sequences in the waa (core OS biosynthesis) gene operon were used to develop a PCR-based system that facilitated unequivocal determination of the core OS types in isolates of E. coli. Expand
Involvement of waaY, waaQ, andwaaP in the Modification of Escherichia coliLipopolysaccharide and Their Role in the Formation of a Stable Outer Membrane*
TLDR
The waaY, waaQ, and waaP genes were independently mutated by insertion of a non-polar antibiotic resistance cassette, and the structures of the resulting mutant core oligosaccharides were determined by chemical analyses and phosphorus-nuclear magnetic resonance spectroscopy. Expand
Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine
TLDR
Mutation into the pyoverdine‐producing strain ML5087 resulted in poor growth of the mutant in an iron‐limited medium, while mutants expressing ORFAB constitutively were constitutive for py overdine and ferripyoverdines receptor production suggesting that components of the pyoversized iron‐transport system are co‐regulated with OrFAB. Expand
Cloning and nucleotide sequence analysis of the ferripyoverdine receptor gene fpvA of Pseudomonas aeruginosa
TLDR
N-terminal amino acid sequence analysis of the purified ferripyoverdine receptor confirmed fpvA as the receptor gene, and indicated that the receptor is initially synthesized as a precursor with a signal sequence of 27 amino acids which is cleaved to yield the mature protein. Expand
Characterization of staphyloferrin A biosynthetic and transport mutants in Staphylococcus aureus
TLDR
It is provided here that HtsABC, along with the FhuC ATPase, is required for the uptake of staphyloferrin A, a polycarboxylate siderophore, and crystal structure of apo‐HtsA was determined and identified a large positively charged region in the substrate‐binding pocket, in agreement with a role in binding of anionic staphylloferrins A. Expand
Demonstration of the Iron-regulated Surface Determinant (Isd) Heme Transfer Pathway in Staphylococcus aureus*
TLDR
Experimental findings corroborate the heme transfer model first proposed by the Schneewind group and show that heme transport from the wall-anchored IsdH/IsdB proteins proceeds directly to IsdE at the membrane and, for this to occur, specific protein-protein interactions must take place. Expand
Haem recognition by a Staphylococcus aureus NEAT domain
TLDR
It is shown that overexpression of IsdA in S. aureus enhances growth and an inactivation mutant of IsDA has a growth defect, compared with wild type, when grown in media containing haem as the sole iron source. Expand
Cloning and sequence analysis of a gene (pchR) encoding an AraC family activator of pyochelin and ferripyochelin receptor synthesis in Pseudomonas aeruginosa
TLDR
A role for PchR as a transcriptional activator of pyochelin and ferripyochelin receptor synthesis in P. aeruginosa is consistent with a role for this mutant obtained by in vitro mutagenesis and gene replacement. Expand
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