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Distribution of phosphodiesterase I in normal human tissues.
Phosphodiesterase I (PDE I) is an exonuclease capable of hydrolyzing a variety of phosphate ester and pyrophosphate bonds. Cell fractionation and histochemical studies in animal tissues haveExpand
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Structure of boromycin.
The structure II of boromycin C45H74BNO15 (cf. [2]) has been established by combination of chemical evidence with X-ray analysis. The antibiotic is a D-valine ester of a Boeseken-complex of boricExpand
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Insulin receptor monoclonal antibodies that mimic insulin action without activating tyrosine kinase.
HTC rat hepatoma cells were transfected with human insulin receptor cDNA to a level of 40,000 receptors/cell. In these cells, as well as in nontransfected cells, insulin stimulated the uptake ofExpand
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Localization of the insulin-binding site to the cysteine-rich region of the insulin receptor alpha-subunit.
Affinity-purified insulin receptor was photoaffinity labeled with a cleavable radioactive insulin photoprobe. Exhaustive digestion of the labeled alpha-subunit with endoproteinase Glu-C produced aExpand
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Comparison of solubilized and purified plasma membrane and nuclear insulin receptors.
Prior studies have detected biochemical and immunological differences between insulin receptors in plasma membranes and isolated nuclei. To further investigate these receptors, they were solubilizedExpand
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Insulin down-regulates insulin receptor number and up-regulates insulin receptor affinity in cells expressing a tyrosine kinase-defective insulin receptor.
We examined the effect of insulin treatment on HTC cells transfected with large numbers of either normal insulin receptors (HTC-IR) or insulin receptors defective in tyrosine kinase (HTC-IR/M-1030).Expand
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Quantitation of the common components of deoxyribonucleic acids by mass spectrometry: application to the analysis of DNAs of unusual composition.
A mass spectral method for the quantitation of the percentages of deoxyadenosine, deoxyguanosine, deoxycytidine, and thymidine in intact DNAs has been devised. Standard curves for each nucleosideExpand
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Mutation of the high cysteine region of the human insulin receptor alpha-subunit increases insulin receptor binding affinity and transmembrane signaling.
Our previous studies indicated that amino acid residues 240-250 in the cysteine-rich region of the human insulin receptor alpha-subunit constitute a site in which insulin binds (Yip, C. C., Hsu, H.,Expand
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Transmembrane signalling by insulin via an insulin receptor mutated at tyrosines 1158, 1162, and 1163.
In order to study the role of tyrosine autophosphorylation in insulin receptor signalling, we investigated a mutant human insulin receptor whereby the three major tyrosine autophosphorylation sitesExpand
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Tissue specificity of human phosphodiesterase. I. Blood serum.
Human blood serum was found to contain two enzymes which hydrolyze various phosphate diester and phosphonate ester bonds. The enzymes were isolated by butanol extraction, ammonium sulfateExpand
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