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Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia
- B. Stevenson, J. Siliciano, M. Mooseker, D. Goodenough
- BiologyJournal of Cell Biology
- 1 September 1986
Immunoblot analysis of Madin-Darby canine kidney cells demonstrates the presence of a polypeptide similar in molecular weight to that detected in liver, suggesting that this protein is potentially a ubiquitous component of all mammalian tight junctions.
Connexin36 Is Essential for Transmission of Rod-Mediated Visual Signals in the Mammalian Retina
Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques
Results demonstrate that gap junction assembly is regulated after arrival of connexin43 at the plasma membrane and is temporally associated with acquisition of insolubility in Triton X-100 and phosphorylation to the connexIn43-P2 form.
Connexin43: a protein from rat heart homologous to a gap junction protein from liver
A nomenclature is proposed which avoids categorization by organ of origin and indicates that the conserved sequences correspond to transmembrane and extracellular regions of the junctional molecules, while the nonconserved sequences correspondto cytoplasmic regions.
Connexins, connexons, and intercellular communication.
Structural-function studies provide a molecular understanding of the significance of connexin diversity and demonstrate the unique regulation of Connexins by tyrosine kinases and oncogenes.
Differential phosphorylation of the gap junction protein connexin43 in junctional communication-competent and -deficient cell lines
A strong correlation is established between the ability of cells to process connexin43 to the P2 form and to produce functional gap junctions and connex in43 phosphorylation may play a functional role in gap junction assembly and/or activity.
Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes
- D. Paul, L. Ebihara, L. Takemoto, K. Swenson, D. Goodenough
- BiologyJournal of Cell Biology
- 2 November 1991
Results suggest that unprocessed Cx46 induces nonselective channels in the oolemma that are voltage dependent and opened by large depolarizations, suggesting substantive lenticular posttranslational processing of the native translation product.
Molecular characterization and tissue distribution of ZO-2, a tight junction protein homologous to ZO-1 and the Drosophila discs-large tumor suppressor protein
Immunohistochemical staining of frozen sections of whole tissue demonstrated that ZO-2 localized to the region of the tight junction in a number of epithelia, including liver, intestine, kidney, testis, and arterial endothelium, suggesting that this protein is a ubiquitous component of the Tight junction.
Connexins Are Critical for Normal Myelination in the CNS
- D. Menichella, D. Goodenough, Erich E. Sirkowski, S. Scherer, D. Paul
- BiologyJournal of Neuroscience
- 2 July 2003
Evidence is provided that gap-junction communication is crucial for normal central myelination in oligodendrocytes and that animals lacking both connexins die by postnatal week 6 from profound abnormalities in central myelin.
Five-hour half-life of mouse liver gap-junction protein
The half-life of a gap-junction polypeptide band migrating at 21,000 Mr on SDS polyacrylamide gels isolated from mouse liver is measured to be 5 h. Two low-molecular wight bands, probably related to…