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Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus.
TLDR
An analysis of total plant RNA sequences using Life Sciences 454 high-throughput sequencing revealed that a vine showing decline symptoms supported a mixed infection that included seven different RNA genomes.
Grapevine leafroll-associated virus 3
TLDR
The characterization of sgRNAs produced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs, and the application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction.
Real-time RT-PCR (TaqMan) assays for the detection of Grapevine Leafroll associated viruses 1-5 and 9.
TLDR
TaqMan RT-PCR was more sensitive than conventional one-step RT- PCR for testing different isolates of these viruses either using RNA or crude tissue extract and showed that when using total RNA extracted either by the Qiagen RNeasy method or by an ABI automated system more isolates were detected.
Nucleotide sequence and rt-PCR detection of a virus associated with grapevine rupestris stem-pitting disease.
TLDR
Sequence analysis indicated that the virus is most similar to apple stem-pitting virus and may be allied with the carla- and potexviruses and grouped with other viruses that infect woody hosts.
Molecular analysis of a California strain of Rupestris stem pitting-associated virus isolated from declining Syrah grapevines
TLDR
The sequence of the genome of a Rupestris stem pitting-associated virus isolated from a declining Syrah grapevine in California, designated the Syrah strain (RSPaV-SY), was determined and the coat protein was the most conserved gene among R SPaV sequences and the replicase was the least conserved genes.
Pathogen testing and certification of Vitis and Prunus species.
TLDR
A strong case is presented for the continued use of slower biological tests in programs requiring high levels of confidence in detection of pathogens that must be excluded from valuable germplasm.
Comparison of low-density arrays, RT-PCR and real-time TaqMan RT-PCR in detection of grapevine viruses.
TLDR
This study includes three new TaqMan RT-PCR assays that have been developed for GLRaV-2RG, GFkV and ToRSV and have been included in the Taq man RT- PCR and LDA detection.
California mealybugs can spread grapevine leafroll disease
TLDR
This study examined the ability of mealybugs, a putative leafroll vector, to transmit this group of viruses and confirmed that four species found in California — obscure, longtailed, citrus and grape mealybug — can transmit GLRaV-3 isolates.
Grapevine Leafroll: A Complex Viral Disease Affecting a High-Value Fruit Crop.
TLDR
An overview of the state of knowledge on the biology and epidemiology of grapevine leafroll disease is provided and management strategies currently deployed in vineyards are described.
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