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Structural disorder of monomeric α-synuclein persists in mammalian cells
Intracellular aggregation of the human amyloid protein α-synuclein is causally linked to Parkinson’s disease. While the isolated protein is intrinsically disordered, its native structure in mammalianExpand
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Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily.
The RCC1 gene of mammals encodes a guanine nucleotide release protein (GNRP). RCC1 and a homolog in Saccharomyces cerevisiae (MTR1/PRP20/SRM1) have previously been implicated in control of mRNAExpand
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Gadolinium tagging for high-precision measurements of 6 nm distances in protein assemblies by EPR.
Double electron-electron resonance (DEER) distance measurements of a protein complex tagged with two Gd(3+) chelates developed for rigid positioning of the metal ion are shown to deliverExpand
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Gd3+ complexes as potential spin labels for high field pulsed EPR distance measurements.
Pulse electron−electron double resonance distance measurements between two high spin Gd3+ ions in a novel bis-Gd3+ complex involving two pyridine-based gadolinium tetracarboxylate systems linked by aExpand
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Utilizing ESEEM spectroscopy to locate the position of specific regions of membrane-active peptides within model membranes.
Membrane-active peptides participate in many cellular processes, and therefore knowledge of their mode of interaction with phospholipids is essential for understanding their biological function. HereExpand
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W-band orientation selective DEER measurements on a Gd3+/nitroxide mixed-labeled protein dimer with a dual mode cavity.
Double electron-electron resonance (DEER) at W-band (95 GHz) was applied to measure the distance between a pair of nitroxide and Gd(3+) chelate spin labels, about 6 nm apart, in a homodimer of theExpand
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Nanometer-scale distance measurements in proteins using Gd3+ spin labeling.
Methods for measuring nanometer-scale distances between specific sites in proteins are essential for analysis of their structure and function. In this work we introduce Gd(3+) spin labeling forExpand
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HYSCORE and DEER with an upgraded 95GHz pulse EPR spectrometer.
The set-up of a new microwave bridge for a 95 GHz pulse EPR spectrometer is described. The virtues of the bridge are its simple and flexible design and its relatively high output power (0.7 W) thatExpand
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Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd3+ spin labels.
The pulse DEER (Double Electron-Electron Resonance) technique is frequently applied for measuring nanometer distances between specific sites in biological macromolecules. In this work we extend theExpand
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Dynamic Nuclear Polarization in the solid state: a transition between the cross effect and the solid effect.
Proton Dynamic Nuclear Polarization (DNP) experiments were conducted on a 3.4 T homebuilt hybrid pulsed-EPR-NMR spectrometer, on static samples containing 10 mM or 40 mM TEMPOL in frozen glassyExpand
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