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Secretory component production by human bronchial epithelial cells is upregulated by interferon gamma.
TLDR
It is concluded that human bronchial epithelial cells produce secretory component and transcytose dimeric immunoglobulin A in vitro and these processes were apically polarized and upregulated by interferon-gamma. Expand
Polymeric IgA Binding to the Human pIgR Elicits Intracellular Signalling, but Fails to Stimulate pIgR‐Transcytosis
TLDR
In transfected polarized MDCK cells, polymeric immunoglobulin A (pIgA) binding stimulates rabbit pIgR‐transcytosis, owing to phospholipase‐Cγ1 activation and increase of intracellular calcium. Expand
Validation of a new ELISA method for in vitro potency assay of hepatitis B-containing vaccines.
TLDR
It has been demonstrated that the ELISA is suitable for its intended purpose with hepatitis B-containing vaccines from GSK and thus could be used for these vaccines by national control laboratories and authorities. Expand
Antibody against the human J chain inhibits polymeric Ig receptor‐mediated biliary and epithelial transport of human polymeric IgA
TLDR
The data strongly support the crucial role of the J chain of native pIGA in its selective pIgR‐mediated transport into secretions, as suggested long ago by in vitro data only. Expand
In vivo stimulation of polymeric Ig receptor transcytosis by circulating polymeric IgA in rat liver.
Binding of human polymeric IgA ligand to its epithelial cell polymeric Ig receptor, pIgR, has been shown to stimulate pIgR apical transcytosis in an in vitro system, based on polarized confluent MDCKExpand
A case study of development, validation, and acceptance of a non-animal method for assessing human vaccine potency
TLDR
The move from the in vivo potency test toward in vitro potency assays: the in vitro Auszyme test (Abbott Laboratories) and the GSK Biologicals in-house test based upon the inhibition enzyme-linked immunosorbent assay (ELISA) principle are described. Expand
Antibody against the human J chain inhibits polymeric Ig receptor-mediated biliary and epithelial transport of human polymeric IgA.
TLDR
The data strongly support the crucial role of the J chain of native pIgA in its selective pIGR-mediated transport into secretions, as suggested long ago by in vitro data only. Expand
Crucial role of J chain in receptor-mediated epithelial transport of polymeric IgA: in vivo and in vitro data
TLDR
The functional data convincingly confirmed the crucial role of the J chain in the epithelial transport of pIgA and inhibited its rat biliary transport in a dose- and time-dependent manner. Expand
Development and validation of a molecular size distribution method for polysaccharide vaccines.
TLDR
The new method gave comparable results to the reference method, but with less intra- and inter-assay variability, and was shown to be a suitable quality control method for the release and stability follow-up of polysaccharide-containing vaccines. Expand
LackofSC/pIgR-mediated epithelial transport ofa humanpolymeric IgAdevoid of Jchain: invitro andinvivo studies
SUMMARY Threehumanpolymeric IgA(pIgA) myelomaproteins oftetrameric size were compared fortheir J-chain content, their invitro secretory component(SC)-binding ability, andtheir capacity tobeExpand