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Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.
Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids wasExpand
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14-3-3 proteins: potential roles in vesicular transport and Ras signaling in Saccharomyces cerevisiae.
Deletion of the clathrin heavy-chain gene, CHC1, in the budding yeast Saccharomyces cerevisiae results in growth, morphological, and membrane trafficking defects, and in some strains chc1-delta isExpand
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Dynamic and complex transcription factor binding during an inducible response in yeast.
Complex biological processes are often regulated, at least in part, by the binding of transcription factors to their targets. Recently, considerable effort has been made to analyze the binding ofExpand
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Bms1p, a novel GTP-binding protein, and the related Tsr1p are required for distinct steps of 40S ribosome biogenesis in yeast.
Bms1p and Tsr1p define a novel family of proteins required for synthesis of 40S ribosomal subunits in Saccharomyces cerevisiae. Both are essential and localize to the nucleolus. Tsr1p shares twoExpand
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Loss of ypk1 function causes rapamycin sensitivity, inhibition of translation initiation and synthetic lethality in 14-3-3-deficient yeast.
14-3-3 proteins bind to phosphorylated proteins and regulate a variety of cellular activities as effectors of serine/threonine phosphorylation. To define processes requiring 14-3-3 function in yeast,Expand
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Phage ESCape: an emulsion-based approach for the selection of recombinant phage display antibodies.
Antibody phage display technology is a well established method for selecting specific antibodies against desired targets. Although phage display is the most widely used method of generating syntheticExpand
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inducible response in yeast Dynamic and complex transcription factor binding during an Material Supplemental
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