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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Expand
Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
The 5'----3' exonuclease activity of the thermostable enzyme Thermus aquaticus DNA polymerase may be employed in a polymerase chain reaction product detection system to generate a specific detectableExpand
Three cdg Operons Control Cellular Turnover of Cyclic Di-GMP in Acetobacter xylinum: Genetic Organization and Occurrence of Conserved Domains in Isoenzymes
  • R. Tal, Hing C. Wong, +11 authors M. Benziman
  • Medicine, Biology
  • Journal of bacteriology
  • 1 September 1998
Genetic disruption analyses demonstrated a physiological hierarchy among the cdg operons, such that cdg1 contributes 80% of cellular DGC and PDEA activities and cdg2 and 5%, respectively, demonstrating that c-di-GMP controls this process. Expand
Recent advances in the polymerase chain reaction
Progresses ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences and some recent developments in instrumentation, methodology, and applications of the PCR are presented. Expand
PCR protocols: a guide to methods and applications.
Basic Methodology: M.A. Innis and D.F. Frohman, RACE: Rapid Amplification of cDNA Ends, and RNA Processing: Apo-B.R. Kwok, Procedure to Minimuze PCR-Product Carry-Over. Expand
Genetic organization of the cellulose synthase operon in Acetobacter xylinum.
Results from genetic complementation tests and gene disruption analyses demonstrate that all four genes in the operon are required for maximal bacterial cellulose synthesis in A. xylinum. Expand
DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.
This work modified the polymerase chain reaction (PCR) conditions for direct DNA sequencing of asymmetric PCR products without intermediate purification by using Taq DNA polymerase, and presented protocols that produce readable extension products greater than 1000 bases having uniform band intensities. Expand
Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases
Biochemical studies on extracts of strains carrying combinations of these aminotransferase mutations confirm the existence of two distinct enzymes with overlapping specificities for the alpha-keto acid analogues of tyrosine, phenylalanine, and aspartate. Expand
Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.
The cloning and expression of Taq DNA polymerase in Escherichia coli is reported and appropriate portions of the insert from a lambda Ch35 library candidate are subcloned and expressed to yield thermostable, active, truncated, or full-length forms of the protein in E. coli under control of the lac promoter. Expand