• Publications
  • Influence
The MutT Proteins or “Nudix” Hydrolases, a Family of Versatile, Widely Distributed, “Housecleaning” Enzymes*
This short review summarizes the present knowledge of those reactions catalyzed by proteins harboring the MutT signature sequence and calls attention to a unique and versatile nucleotide binding and catalytic site. Expand
Studies on the ADP-ribose Pyrophosphatase Subfamily of the Nudix Hydrolases and Tentative Identification of trgB, a Gene Associated with Tellurite Resistance*
A highly specific ADP-ribose pyrophosphatase gene from the archaebacterium, Methanococcus jannaschii, introduced into E. coli, increased the transformant's tolerance to potassium tellurite. Expand
DNA primases.
DNA primases are enzymes whose continual activity is required at the DNA replication fork. They catalyze the synthesis of short RNA molecules used as primers for DNA polymerases. Primers areExpand
Ivermectin is a potent inhibitor of flavivirus replication specifically targeting NS3 helicase activity: new prospects for an old drug.
Ivermectin, a broadly used anti-helminthic drug, proved to be a highly potent inhibitor of YFV replication and inhibited, although less efficiently, the replication of several other flaviviruses, i.e. dengue fever, Japanese encephalitis and tick-borne encephalopathy viruses. Expand
Cloning, Purification, and Properties of a Novel NADH Pyrophosphatase
An Escherichia coli open reading frame containing significant homology to the active site of the MutT enzyme codes for a novel dinucleotide pyrophosphatase, which catalyzes the hydrolysis of a broad range of dinucleophosphates, but uniquely prefers the reduced form of NADH. Expand
Orf186 Represents a New Member of the Nudix Hydrolases, Active on Adenosine(5′)triphospho(5′)adenosine, ADP-ribose, and NADH*
orf186, a new member of the Nudix hydrolase family of genes, has been cloned and expressed, and the protein has been purified and identified as an enzyme highly specific for compounds of ADP. ItsExpand
Cyclophilin B stimulates RNA synthesis by the HCV RNA dependent RNA polymerase.
Cyclophilins are cellular peptidyl isomerases that have been implicated in regulating hepatitis C virus (HCV) replication and the effects of purified CypB proteins on the enzymatic activity of NS5B were examined. Expand
The hepatitis C virus NS3 protein: a model RNA helicase and potential drug target.
  • D. Frick
  • Biology, Medicine
  • Current issues in molecular biology
  • 2007
Compounds recently reported to inhibit HCV helicase will be useful for elucidating the role of a helicase in positive-sense single-stranded RNA virus replication and might serve as templates for the design of novel antiviral drugs. Expand
Solution structure of the quaternary MutT-M2+-AMPCPP-M2+ complex and mechanism of its pyrophosphohydrolase action.
On the basis of the structure of the quaternary complex, a mechanism of the MutT reaction is proposed which is qualitatively and quantitatively consistent with kinetic and mutagenesis studies and suggested that similar mechanisms may be operative for other enzymes that catalyze substitution at P(beta) of NTP substrates. Expand
Analysis of the Evolutionary Forces in an Immunodominant CD8 Epitope in Hepatitis C Virus at a Population Level
A complex interaction of multiple forces shaping the evolution of HCV in which immune pressure both within the individual and also at the population level in addition to functional constraints are important contributing factors is suggested. Expand