A predominantly neolithic origin for Y-chromosomal DNA variation in North Africa.
The Neolithic transition in this part of the world was accompanied by demic diffusion of Afro-Asiatic-speaking pastoralists from the Middle East, and it is suggested that the North African pattern of Y-chromosomal variation is largely of Neolithic origin.
A strategy for high-level expression of soluble and functional human interferon alpha as a GST-fusion protein in E. coli.
- Imen Rabhi-Essafi, Amine Sadok, N. Khalaf, D. Fathallah
- BiologyProtein engineering, design & selection : PEDS
- 1 May 2007
These data are the first to show that high yield production of soluble and functional rhIFNalpha2b tagged with GST can be achieved in E. coli, without optimization of any step of the process.
A novel silicon nitride biosensor for specific antibody-antigen interaction
A novel simple and rapid PCR-based site-directed mutagenesis method
This procedure was used efficiently to introduce substitutions, deletions, and insertions in the DNA sequences coding for a recombinant form of antibody 107 specific of the human CR3 molecule, the rat α integrin CD11b A-domain and the human CD8β cloned in pPICZαB, pGEX-2T, and CDM8 expression vectors, respectively.
Binding Affinity of Metal Ions to the CD11b A-domain Is Regulated by Integrin Activation and Ligands*
- K. Ajroud, T. Sugimori, W. Goldmann, D. Fathallah, J. Xiong, M. Arnaout
- Biology, ChemistryJournal of Biological Chemistry
- 11 June 2004
The affinity of metal ions to integrins is itself regulated by the activation state of these receptors and by certain ligands, elucidate a new level of regulation of the integrin-metal-ligand ternary complex and help explain some of the discrepant effects of Ca2+ on integrin -ligand interactions.
Carbonic anhydrase II (CA II) deficiency in Maghrebian patients: evidence for founder effect and genomic recombination at the CA II locus
- D. Fathallah, M. Bejaoui, D. Lepaslier, Khelifa Chater, W. Sly, K. Dellagi
- BiologyHuman Genetics
- 18 April 1997
A founder effect to explain the common CA II deficiency allele in this population of Tunisian families is supported, with the relatively high recombination frequency suggests the presence of a hot spot for recombination or gene conversion at the CA II locus.
Enhanced Patient Serum Immunoreactivity to Recombinant Mycobacterium tuberculosis CFP32 Produced in the Yeast Pichia pastoris Compared to Escherichia coli and Its Potential for Serodiagnosis of…
- C. Benabdesselem, D. Fathallah, R. Barbouche
- Biology, MedicineJournal of Clinical Microbiology
- 1 September 2006
The trans-production of rCFP32 in P. pastoris significantly improved the serologic detection of CFP32-specific antibodies in patient sera, thereby offering a new, possibly better, modality for producing antigens of diagnostic potential for use in the development of immunoassays for both TB and other infectious diseases.
Expression in Pichia pastoris of a recombinant scFv form of MAb 107, an anti human CD11b integrin antibody
Expression by Streptomyces lividans of the Rat α Integrin CD11b A-Domain as a Secreted and Soluble Recombinant Protein
- D. Ayadi, H. Chouayekh, Sonda Mhiri, K. Zerria, D. Fathallah, S. Bejar
- Biology, EngineeringJournal of Biomedicine and Biotechnology
- 27 December 2006
The expression and secretion of the rat CD11b A-domain is reported using the same LSSP and S. lividans as host strain to support the capability of Streptomyces to produce heterologous recombinant proteins as soluble secreted form using the “LSSP” synthetic signal peptide.
Use of Taguchi's methods as a basis to optimize hybridoma cell line growth and antibody production in a spinner flask
This study emphasizes the value of using Taguchi’s methods as a basis for optimization of mAb production from a hybridoma culture, in cost effective and significantly less labor intensive ways.