• Publications
  • Influence
Efficient TALEN-mediated gene knockout in livestock
TLDR
TALENs appear to represent a highly facile platform for the modification of livestock genomes for both biomedical and agricultural applications.
In vivo Genome Editing Using High Efficiency TALENs
TLDR
Improvements in artificial transcription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zebrafish genome editing and functional genomic applications and offer the potential to model genetic variation as well as to generate targeted conditional alleles.
Efficient nonmeiotic allele introgression in livestock using custom endonucleases
TLDR
It is demonstrated that genetic variants can be directly introgressed into livestock genomes using a modified transcription activator-like effector nuclease system and expanded the livestock gene editing toolbox to include TALEN and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-stimulated homology-directed repair (HDR).
TALEN-based gene correction for epidermolysis bullosa.
TLDR
This study provides proof-of-concept for TALen-mediated in situ correction of an endogenous patient-specific gene mutation and used an unbiased screen for comprehensive TALEN target mapping that will cooperatively facilitate translational application.
Genome edited sheep and cattle
TLDR
Zygote injection of TALEN mRNA can also produce gene-edited cattle and sheep and provides a practical alternative to somatic cell nuclear transfer for gene knockout or introgression of desirable alleles into a target breed/genetic line.
Live pigs produced from genome edited zygotes
TLDR
It is demonstrated for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs.
Targeting G with TAL Effectors: A Comparison of Activities of TALENs Constructed with NN and NK Repeat Variable Di-Residues
TLDR
It is shown that TAL effector nucleases (TALENs) made with NK to specify G have less activity than their NN-containing counterparts: fourteen of fifteen TALEN pairs made with NN showed more activity in a yeast recombination assay than otherwise identical TALens made withNK.
Targeting DNA With Fingers and TALENs
TLDR
Functional and design characteristics of ZFNs and TALENs are described and their expanding role as tools for research and gene therapy is discussed and they are widely considered the most mature nuclease technology in the gene therapy field.
Enzymatic engineering of the porcine genome with transposons and recombinases
TLDR
This study has demonstrated for the first time that transposons and recombinases are capable of mobilizing DNA into and out of the porcine genome in a precise and efficient manner.
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