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SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells
This screen identified the signal-regulatory protein alpha (SIRPA) as a marker expressed specifically on cardiomyocytes derived from hESCs and human induced pluripotent stem cells (hiPSCs), and PECAM, THY1, PDGFRB and ITGA1 as markers of the nonmyocyte population.
Differentiation of human embryonic stem cells and induced pluripotent stem cells to cardiomyocytes: a methods overview.
- C. Mummery, Jianhua Zhang, Elizabeth S. Ng, D. Elliott, A. Elefanty, T. Kamp
- BiologyCirculation research
- 20 July 2012
The basic biology underlying the differentiation of pluripotent cells to cardiac lineages is reviewed and current state-of-the-art protocols are described, as well as ongoing refinements.
NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes
NKX2-5 eGFP+ cells are used to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages and facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells and cardiomyocytes and the standardization of differentiation protocols.
Cardiac T-box factor Tbx20 directly interacts with Nkx2-5, GATA4, and GATA5 in regulation of gene expression in the developing heart.
Four distinct pathways of hemoglobin uptake in the malaria parasite Plasmodium falciparum
- D. Elliott, M. T. McIntosh, K. Joiner
- Biology, MedicineProceedings of the National Academy of Sciences
- 19 February 2008
Using serial thin-section electron microscopy and three-dimensional reconstruction, this work demonstrates four independent, but partially overlapping, hemoglobin-uptake processes distinguishable temporally, morphologically, and pharmacologically in Plasmodium.
Independent Regulation of Synaptic Size and Activity by the Anaphase-Promoting Complex
Cardiac Septal and Valvular Dysmorphogenesis in Mice Heterozygous for Mutations in the Homeobox Gene Nkx2-5
In a proportion of neonatal heterozygotes, as well as in adults with ASD, it was found that the size of the foramen ovale was significantly enlarged and altered in shape, potentially exposing the normally thin septum primum to excessive hemodynamic forces.
Divergent roles for NK-2 class homeobox genes in cardiogenesis in flies and mice.
The results suggest that, while tin and Nkx2-5 show close functional kinship, their mode of deployment in cardiogenesis has diverged possibly because of differences in their interactions with accessory factors.
Atrial-like cardiomyocytes from human pluripotent stem cells are a robust preclinical model for assessing atrial-selective pharmacology
It is demonstrated that hESC‐atrial CMs are a robust model for pre‐clinical testing to assess atrial selectivity of novel antiarrhythmic drugs.
Apolipoproteins in the brain: implications for neurological and psychiatric disorders
An up-to-date assessment of the major apolipoproteins found in the brain (ApoE, ApOJ, ApoD and ApoA-I), covering their proposed roles and the factors influencing their level of expression is provided, placed on associations with neurological and psychiatric disorders.