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High Rates of Actin Filament Turnover in Budding Yeast and Roles for Actin in Establishment and Maintenance of Cell Polarity Revealed Using the Actin Inhibitor Latrunculin-A
We report that the actin assembly inhibitor latrunculin-A (LAT-A) causes complete disruption of the yeast actin cytoskeleton within 2–5 min, suggesting that although yeast are nonmotile, their actin
cdc12p, a Protein Required for Cytokinesis in Fission Yeast, Is a Component of the Cell Division Ring and Interacts with Profilin
A model in which ring assembly originates from a single point on the cortex and in which a molecular pathway for the functions of cytokinesis proteins is suggested is presented.
Fluorescence microscopy methods for yeast.
The Saccharomyces cerevisiae Mob2p–Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell–specific localization of Ace2p transcription factor
Data indicate that Mob2p–Cbk1p functions to maintain polarized cell growth, prevent the nuclear export of Ace2p from the daughter cell nucleus after mitotic exit, and coordinate Ace1p-dependent transcription with MEN activation.
Harnessing actin dynamics for clathrin-mediated endocytosis
Live-cell imaging studies are shedding light on the order and timing of the molecular events and mechanisms of actin function during endocytosis.