• Publications
  • Influence
Matrix Elasticity Directs Stem Cell Lineage Specification
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types. Expand
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues. Expand
Nuclear Lamin-A Scales with Tissue Stiffness and Enhances Matrix-Directed Differentiation
Introduction Tissues can be soft like brain, bone marrow, and fat, which bear little mechanical stress, or stiff like muscle, cartilage, and bone, which sustain high levels of stress. SystematicExpand
Substrate compliance versus ligand density in cell on gel responses.
A central structural role for the cytoskeleton is hypothesize in driving the membrane outward during spreading whereas adhesion reinforces the spreading. Expand
Growth Factors, Matrices, and Forces Combine and Control Stem Cells
Multifaceted technologies are increasingly required to produce and interrogate cells ex vivo, to build predictive models, and, ultimately, to enhance stem cell integration in vivo for therapeutic benefit. Expand
Myotubes differentiate optimally on substrates with tissue-like stiffness
Contractile myocytes provide a test of the hypothesis that cells sense their mechanical as well as molecular microenvironment, altering expression, organization, and/or morphology accordingly, and have major implications for in vivo introduction of stem cells into diseased or damaged striated muscle of altered mechanical composition. Expand
Polymersomes: tough vesicles made from diblock copolymers.
The results suggest a new class of synthetic thin-shelled capsules based on block copolymer chemistry, and both the membrane bending and area expansion moduli of electroformed polymersomes (polymer-based liposomes) fell within the range of lipid membrane measurements. Expand
Shape effects of filaments versus spherical particles in flow and drug delivery.
Highly stable, polymer micelle assemblies known as filomicelles are used to compare the transport and trafficking of flexible filaments with spheres of similar chemistry and show that long-circulating vehicles need not be nanospheres. Expand
Physical plasticity of the nucleus in stem cell differentiation
Micromanipulation methods show that nuclei in human embryonic stem cells are highly deformable and stiffen 6-fold through terminal differentiation, and that nucleo-skeletal components in human adult stem cells possess an intermediate stiffness and deform irreversibly. Expand