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PKCη regulates occludin phosphorylation and epithelial tight junction integrity
  • T. Suzuki, B. Elias, +6 authors R. Rao
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 6 January 2009
TLDR
Evidence is presented that PKCη phosphorylates occludin on threonine residues (T403 and T404) and plays a crucial role in the assembly and/or maintenance of TJs in Caco-2 and MDCK cell monolayers. Expand
Phosphorylation of Tyr-398 and Tyr-402 in Occludin Prevents Its Interaction with ZO-1 and Destabilizes Its Assembly at the Tight Junctions*
TLDR
A unique motif in the occludin sequence is revealed that is involved in the regulation of ZO-1 binding by reversible phosphorylation of specific Tyr residues. Expand
Gram‐positive bacteria produce membrane vesicles: Proteomics‐based characterization of Staphylococcus aureus‐derived membrane vesicles
TLDR
It is found for the first time that Gram‐positive bacteria naturally produce MVs into the extracellular milieu and these observations suggest that the secretion of MVs is an evolutionally conserved, universal process that occurs from simple organisms to complex multicellular organisms. Expand
Direct quantitative analysis of lysophosphatidic acid molecular species by stable isotope dilution electrospray ionization liquid chromatography-mass spectrometry.
TLDR
A stable-isotope dilution, liquid chromatography-mass spectrometry assay for the direct quantitative analysis of 1-acyl-LPA, using a deuterium-labeled internal standard, LPA, and a single liquid-liquid extraction with acidic butanol that allows >95% recovery of LPA. Expand
Novel molecular signaling and classification of human clinically nonfunctional pituitary adenomas identified by gene expression profiling and proteomic analyses.
TLDR
Different gene and protein expression patterns in adenomas are shown, providing new insight into the pathogenesis and molecular classification of nonfunctional pituitary adenoma, and suggest that therapeutic targeting of the Notch pathway could be effective for these tumors. Expand
EVpedia: an integrated database of high-throughput data for systemic analyses of extracellular vesicles
TLDR
Evolpedia is presented, which is an integrated database of high-throughput datasets from prokaryotic and eukaryotic extracellular vesicles that might serve as a fundamental repository to stimulate the advancement of extrace cellular vesicle studies and to elucidate the novel functions of these complexextracellular organelles. Expand
EVpedia: a community web portal for extracellular vesicles research
TLDR
An improved version of EVpedia, a public database for EVs research, is presented, which contains a database of publications and vesicular components, identification of orthologous vesicle components, bioinformatic tools and a personalized function. Expand
Proteomics analysis of human cerebrospinal fluid.
TLDR
The variety of analytical methods that have been used for proteomics analysis of CSF, including sample preparation, two-dimensional liquid and gel electrophoresis, mass spectrometry, bioinformatics, and non-gel methods are reviewed. Expand
Acetaldehyde dissociates the PTP1B-E-cadherin-beta-catenin complex in Caco-2 cell monolayers by a phosphorylation-dependent mechanism.
TLDR
Results show that acetaldehyde induces disruption of interactions between E-cadherin, beta-catenin and PTP1B by a phosphorylation-dependent mechanism. Expand
Preparation of stable isotope-incorporated peptide internal standards for field desorption mass spectrometry quantification of peptides in biologic tissue.
TLDR
This scheme represents the first fast and facile preparation of a stable isotope-incorporated peptide internal standard for use in measuring peptides in biologic extracts and is readily extended to any peptide with a free carboxyl group. Expand
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