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Plasticity of genetic interactions in metabolic networks of yeast
The majority of synthetic genetic interactions are restricted to certain environmental conditions, partly because of the lack of compensation under some (but not all) nutrient conditions, and this work offers a unified framework for the evolution of environmental adaptation and mutational robustness. Expand
Sequencing and Characterisation of Rearrangements in Three S. pastorianus Strains Reveals the Presence of Chimeric Genes and Gives Evidence of Breakpoint Reuse
It is shown that 28/30 S. cerevisiae- S. eubayanus recombination breakpoints are located within genic regions, generating chimeric genes, and evidence for the reuse of two breakpoints, located in HSP82 and KEM1, in strains of proposed independent origin. Expand
Engineering evolution to study speciation in yeasts
Experiments are reported that take an interventionist, rather than a retrospective approach to studying speciation, by reconfiguring the Saccharomyces cerevisiae genome so that it is collinear with that of SacCharomyces mikatae, and it is demonstrated that this imposed genomicCollinearity allows the generation of interspecific hybrids that produce a large proportion of spores that are viable, but extensively aneuploid. Expand
Exploring redundancy in the yeast genome: an improved strategy for use of the cre-loxP system.
A method for performing rapid multiple gene disruptions in Saccharomyces cerevisiae, which significantly accelerates and facilitates the functional analysis process and is particularly useful for studying gene families in either laboratory or industrial yeast strains. Expand
New generation of loxP‐mutated deletion cassettes for the genetic manipulation of yeast natural isolates
A new generation of dominant cassettes are constructed, with mutated loxP sites (loxLE and lox2272) and selectable drug markers, to create heterothallic strains and auxotrophic mutants without incurring in the risk of generating chromosomal rearrangements. Expand
Intron Evolution in Saccharomycetaceae
Analysis of a comprehensive set of 250 orthologous introns in the 20 species that comprise the Saccharomycetaceae finds evidence that loss of a small number of introns is mediated by micro-homology, and that the number of intron losses is diminished in yeast species that have lost the microhomology end joining and nonhomologous end joining machinery. Expand
The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
No correlation was found between G+C content and gene density along the chromosome, and their variations are random, so accurate verification procedures demonstrate that there are less than two errors per 10,000 base pairs in the published sequence. Expand
Genome‐wide analysis and proteomic studies reveal APE1/Ref‐1 multifunctional role in mammalian cells
Overall, the data show that APE1 acts as a hub in coordinating different and vital functions in mammalian cells, highlighting the molecular determinants of the multifunctional nature ofAPE1 protein. Expand
Genome-wide analysis of yeast stress survival and tolerance acquisition to analyze the central trade-off between growth rate and cellular robustness
A genome-wide analysis of the acquisition of stress cross-tolerance shows that reduction of growth rate is an important determinant of severe stress survival. Cellular functions important for theExpand
Cellular Processes and Pathways That Protect Saccharomyces cerevisiae Cells against the Plasma Membrane-Perturbing Compound Chitosan
It is proposed that global fitness analysis of yeast in combination with T-profiler is a powerful tool to identify specific cellular processes and pathways that are required for survival under stress conditions. Expand