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Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae.
TLDR
The development of a useful method composed of a set of six multiplex PCRs and one simplex PCR for the rapid screening of the most frequently encountered beta-lactamases is reported. Expand
Genomic Definition of Hypervirulent and Multidrug-Resistant Klebsiella pneumoniae Clonal Groups
We created a Web-accessible genome database to enable rapid extraction of genotype, virulence, and resistance information from sequences.
Phenotypic Screening of Carbapenemases and Associated β-Lactamases in Carbapenem-Resistant Enterobacteriaceae
TLDR
A new strategy to detect carbapenemases (MBL- and KPC-type) and associated mechanisms of β-lactam resistance (ESBL or class A β- lactamase 2b) by the use of inhibitor-impregnated agar is proposed. Expand
wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella Strains
TLDR
Wzi sequencing is a rapid and simple method for the determination of the K types of most K. pneumoniae clinical isolates and the prediction of K type once the wzi allele was known was 94%. Expand
Emerging Severe and Fatal Infections Due to Klebsiella pneumoniae in Two University Hospitals in France
TLDR
This report shows their emergence in France, with 12 cases observed during a 2-year period in two university hospitals, three of which were associated with pneumonia, whereas seven liver abscess cases were caused by K1 strains of ST23. Expand
First outbreak of multidrug-resistant Klebsiella pneumoniae carrying blaVIM-1 and blaSHV-5 in a French university hospital.
TLDR
The potential risk of spread of multiresistant bacteria with international transfer of patients is confirmed, with eight imipenem-resistant isolates of Klebsiella pneumoniae involved in an outbreak in a French teaching hospital confirmed. Expand
Clinical features of Clostridium difficile-associated diarrhoea due to binary toxin (actin-specific ADP-ribosyltransferase)-producing strains.
TLDR
There could be a correlation between the production of binary toxin and the severity of diarrhoea, and cases and controls did not differ significantly for sex, age, previous administration of antibiotics or frequency of endoscopic examination. Expand
Molecular characterisation by PCR-restriction fragment length polymorphism of TEM beta-lactamases.
TLDR
The combination of polymerase chain reaction-restriction fragment length polymorphism and plasmid analysis allowed us to investigate nosocomial outbreaks due to clinical isolates of multi-resistant Klebsiella pneumoniae in three hospitals. Expand
Phylogeny and Identification of Pantoea Species and Typing of Pantoea agglomerans Strains by Multilocus Gene Sequencing
TLDR
A multilocus gene sequencing method is provided that is a powerful tool for Pantoea species delineation and identification and for strain tracking and reveals the very high discrimination power of this method for strain typing and population structure in this species. Expand
Outbreak of multiply resistant enterobacteriaceae in an intensive care unit: epidemiology and risk factors for acquisition.
TLDR
It is concluded that ESBLPE acquisition depends on length of stay in the ICU and the use of invasive procedures, and Colonization is a prerequisite for infection. Expand
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