• Publications
  • Influence
Rapid leukocyte migration by integrin-independent flowing and squeezing
TLDR
It is shown here that functional integrins do not contribute to migration in three-dimensional environments, and these cells migrate by the sole force of actin-network expansion, which promotes protrusive flowing of the leading edge. Expand
Structural basis for vinculin activation at sites of cell adhesion
TLDR
It is shown that conformational changes in the head, tail and proline-rich domains are linked structurally and thermodynamically, and proposed a combinatorial pathway to activation that ensures that vinculin is activated only at sites of cell adhesion when two or more of its binding partners are brought into apposition. Expand
Talin depletion reveals independence of initial cell spreading from integrin activation and traction
TLDR
It is suggested that talin is not required for initial cell spreading, however, talin provides the important mechanical linkage between ligand-bound integrins and the actin cytoskeleton required to catalyse focal adhesion-dependent pathways. Expand
Structural determinants of integrin recognition by talin.
TLDR
The crystal structure of the principal integrin binding and activating fragment of talin is reported, alone and in complex with fragments of the beta 3 integrin tail, providing structural paradigms for integrin linkage to the cell interior. Expand
Talins and kindlins: partners in integrin-mediated adhesion
TLDR
New data reveal the domain structure of full-length talin, provide insights into talin-mediated integrin activation and show that RIAM recruits talin to the plasma membrane, whereas vinculin stabilizes talin in cell–matrix junctions. Expand
Crystal Structure of the Vinculin Tail Suggests a Pathway for Activation
TLDR
It is shown that the C-terminal arm is required for binding to acidic phospholipids but not to actin, and that binding either ligand induces conformational changes that may represent the first step in activation. Expand
The structure and regulation of vinculin.
TLDR
The finding that vinculin modulates the signalling pathways involved in apoptosis suggests that additional roles for vINCulin remain to be discovered. Expand
The structure of an integrin/talin complex reveals the basis of inside‐out signal transduction
TLDR
This work reports the first structure of talin bound to an authentic full‐length β integrin tail and identifies a positively charged surface on the talin F2 domain that precisely orients talin to disrupt the heterodimeric integrin transmembrane (TM) complex. Expand
Role of vinculin in regulating focal adhesion turnover.
TLDR
The results suggest that vinculin inhibits cell migration by stabilising FAs, and that binding of inositol phospholipids to Vt plays an important role in FA turnover. Expand
Calpain-mediated proteolysis of talin regulates adhesion dynamics
TLDR
It is demonstrated that disassembly of other adhesion components, including paxillin, vinculin and zyxin, is also dependent on the ability of calpain to cleave talin, suggesting a general role for talin proteolysis in regulating adhesion turnover. Expand
...
1
2
3
4
5
...