• Publications
  • Influence
MicroRNAs expressed by herpes simplex virus 1 during latent infection regulate viral mRNAs
Herpesviruses are characterized by their ability to maintain life-long latent infections in their animal hosts. However, the mechanisms that allow establishment and maintenance of the latent stateExpand
  • 601
  • 53
  • PDF
A selective inhibitor of eIF2alpha dephosphorylation protects cells from ER stress.
Most protein phosphatases have little intrinsic substrate specificity, making selective pharmacological inhibition of specific dephosphorylation reactions a challenging problem. In a screen for smallExpand
  • 682
  • 50
Enzymatic Amplification of DNA by PCR: Standard Procedures and Optimization
This unit describes a method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequenceExpand
  • 110
  • 44
  • PDF
A Selective Inhibitor of eIF2α Dephosphorylation Protects Cells from ER Stress
Most protein phosphatases have little intrinsic substrate specificity, making selective pharmacological inhibition of specific dephosphorylation reactions a challenging problem. In a screen for smallExpand
  • 645
  • 23
Numerous Conserved and Divergent MicroRNAs Expressed by Herpes Simplex Viruses 1 and 2
ABSTRACT Certain viruses use microRNAs (miRNAs) to regulate the expression of their own genes, host genes, or both. Previous studies have identified a limited number of miRNAs expressed by herpesExpand
  • 137
  • 19
  • PDF
Thymidine kinase-negative herpes simplex virus mutants establish latency in mouse trigeminal ganglia but do not reactivate.
Herpes simplex virus infection of mammalian hosts involves lytic replication at a primary site, such as the cornea, translocation by axonal transport to sensory ganglia and replication, and latentExpand
  • 347
  • 18
  • PDF
The Human Cytomegalovirus UL97 Protein Kinase, an Antiviral Drug Target, Is Required at the Stage of Nuclear Egress
ABSTRACT Human cytomegalovirus encodes an unusual protein kinase, UL97, that activates the established antiviral drug ganciclovir and is specifically inhibited by a new antiviral drug, maribavir. WeExpand
  • 217
  • 16
Prediction and Identification of Herpes Simplex Virus 1-Encoded MicroRNAs
ABSTRACT MicroRNAs (miRNAs) are key regulators of gene expression in higher eukaryotes. Recently, miRNAs have been identified from viruses with double-stranded DNA genomes. To attempt to identifyExpand
  • 223
  • 16
  • PDF
Isolation and characterization of herpes simplex virus mutants containing engineered mutations at the DNA polymerase locus.
We have derived Vero cell lines containing the herpes simplex virus DNA polymerase (pol) gene that complement temperature-sensitive pol mutants. These cell lines were used to recover virusesExpand
  • 58
  • 15
The crystal structure of an unusual processivity factor, herpes simplex virus UL42, bound to the C terminus of its cognate polymerase.
Herpes simplex virus DNA polymerase is a heterodimer composed of a catalytic subunit, Pol, and an unusual processivity subunit, UL42, which, unlike processivity factors such as PCNA, directly bindsExpand
  • 130
  • 15
...
1
2
3
4
5
...