• Publications
  • Influence
A mutational assay system using the thymidine kinase locus in mouse lymphoma cells.
An estimate can be made of the relative mutagenicities of various treatments of the TK locus and the observed induced mutation rates with the spontaneous TK +/− → TK −/− mutation rate. Expand
Validation and characterization of the L5178Y/TK+/- mouse lymphoma mutagen assay system.
Characterization of the TK-/- mutants suggests that two mutagenic mechanisms contribute to their final yield, and is consistent with the induction of slow-growing specific locus mutants by a chromosomal mechanism and their subsequent dilution during this long expression time. Expand
The L5178Y/tk+/- mouse lymphoma specific gene and chromosomal mutation assay a phase III report of the U.S. Environmental Protection Agency Gene-Tox Program.
For most chemicals the L5178Y/tk+/- mouse lymphoma assay is eminently well suited for genotoxicity testing and for predicting the potential for carcinogenicity. Expand
Nonclinical toxicology studies with zidovudine: genetic toxicity tests and carcinogenicity bioassays in mice and rats.
It was concluded that the vaginal tumors seen in the oral carcinogenicity studies were the result of chronic local exposure of the vaginal epithelium to high urine concentrations of ZDV. Expand
Analysis of trifluorothymidine-resistant (TFTr) mutants of L5178Y/TK+/- mouse lymphoma cells.
The inclusion of sigma mutants in the total induced mutant frequency, as well as distinguishing them as a separate subpopulation of TK-deficient mutants, is essential in obtaining maximum utility of the information provided by the L5178Y/TK+/- mouse lymphoma assay. Expand
Specific gene mutations in L5178Y cells in culture.
The correlation of the TK locus assay results with the carcinogenicity data revealed that 2 agents were definite false positives, while benzo[e]pyrene was a questionable false negative, and the assay is of value in the battery approach to mutagenicity/carcinogenicity screening. Expand
Recent developments with the L5178Y TK heterozygote mutagen assay system.
  • D. Clive
  • Chemistry, Medicine
  • Environmental health perspectives
  • 1 December 1973
The presently available mutagen test systems are no longer receiving critical acclaim by sole virtue of their positive response to ethyl methanesulfonate (EMS). Rather, they are being asked to yieldExpand
Molecular aspects of chemical mutagenesis in L5178Y/tk +/- mouse lymphoma cells.
At least three of these mutagens-methotrexate, caffeine, methapyrilene (and possibly procarbazine)--lack structural alerts for DNA reactivity, implying a major class of non-DNA primary targets for mutagenicity in mammalian cells that interact secondarily with the chromosome. Expand