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Point mutations in the transmembrane domain of DjlA, a membrane‐linked DnaJ‐like protein, abolish its function in promoting colanic acid production via the Rcs signal transduction pathway
It is shown that both the TMD and the J‐region are essential for the induction of cps expression observed with the overproduction of DjlA, and proposed that these mutations are affecting specific interactions between the T MD of DjLA and its substrate protein(s), for example RcsC, the membrane sensor kinase partner of the Rcs/B signal transduction pathway.
The chemical basis of serine palmitoyltransferase inhibition by myriocin.
- J. Wadsworth, D. Clarke, +7 authors D. Campopiano
- Chemistry, MedicineJournal of the American Chemical Society
- 11 September 2013
By combining spectroscopy, mass spectrometry, X-ray crystallography, and kinetics, the molecular details of SPT inhibition by myriocin are characterized and this surprising dual mechanism of inhibition rationalizes the extraordinary potency and longevity of myriOCin inhibition.
A novel DnaJ‐like protein in Escherichia coli inserts into the cytoplasmic membrane with a Type III topology
It is proposed that DjlA plays a role in the correct assembly, activity and/or maintenance of a number of membrane proteins, including two‐component signal‐transduction systems.
Genetic Analysis of the RcsC Sensor Kinase from Escherichia coli K-12
- D. Clarke, S. Joyce, C. Toutain, A. Jacq, I. Holland
- Medicine, BiologyJournal of bacteriology
- 15 February 2002
The first genetic data are presented that support the role of RcsC as a hybrid sensor kinase in Escherichia coli.
Structure-Activity Relationships in Defensin Dimers
- D. Campopiano, D. Clarke, +5 authors J. Dorin
- Chemistry, MedicineJournal of Biological Chemistry
- 19 November 2004
This first description of a covalently cross-linked member of thedefensin family provides further evidence that the antimicrobial activity of a defensin is linked to its ability to form stable higher order structures.
Dying and Necrotic Neutrophils Are Anti-Inflammatory Secondary to the Release of α-Defensins1
How human neutrophils dying by apoptosis or necrosis release anti-inflammatory peptides, the α-defensins is described, which effectively inhibits the secretion of multiple proinflammatory cytokines and NO from macrophages, the main innate immune cell found at sites of chronic inflammation.
Analysis and Separation of Residues Important for the Chemoattractant and Antimicrobial Activities of β-Defensin 3*
- K. Taylor, D. Clarke, +10 authors J. Dorin
- Biology, MedicineJournal of Biological Chemistry
- 14 March 2008
The chemoattractant and antimicrobial activities of β-defensins can be separated, and both of these functions are independent of intramolecular disulfide bonds.
Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation.
- J. Lowther, B. A. Yard, +8 authors D. Campopiano
- Chemistry, MedicineMolecular bioSystems
- 1 September 2010
A novel mechanism of CS inactivation of the PLP-dependent enzyme serine palmitoyltransferase (SPT) from Sphingomonas paucimobilis is described, suggesting this occurs by ring opening of the cycloserine ring followed by decarboxylation.
Maturation of McjA precursor peptide into active microcin MccJ25.
In vitro maturation of the microcin precursor peptide into active microcin J25 for the first time is reported and it is shown that the enzymes required for the posttranslational modification of this precursor peptides are associated with the bacterial inner membrane.