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XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan
- D. Chiniquy, Vaishali Sharma, P. Ronald
- BiologyProceedings of the National Academy of Sciences
- 1 October 2012
Insight is provided into grass xylan synthesis and how substitutions may be modified for increased saccharification for biofuel generation and how xylosyltransferase activity onto endogenous acceptors is demonstrated.
Overexpression of a BAHD Acyltransferase, OsAt10, Alters Rice Cell Wall Hydroxycinnamic Acid Content and Saccharification1[C][W][OA]
Results suggest that OsAT10 is a p-coumaroyl coenzyme A transferase involved in glucuronoarabinoxylan modification, an attractive target for improving grass cell wall quality for fuel and animal feed.
Three Novel Rice Genes Closely Related to the Arabidopsis IRX9, IRX9L, and IRX14 Genes and Their Roles in Xylan Biosynthesis
It is demonstrated that the over-expression of the closely related rice genes, in full or partly complement the two well-characterizedArabidopsis irregular xylem (irx) mutants: irx9 and irx14, have similar functions to the Arabidopsis IRX9 and IRX14 genes, respectively.
Inactivation of OsIRX10 leads to decreased xylan content in rice culm cell walls and improved biomass saccharification.
Xylan contributes to the recalcitrance of plant cell walls to enzymatic degradation probably through direct interaction with cellulose microfibrils and also through formation of ferulate cross-links.
pGPro1, a novel binary vector for monocot promoter characterization
The pGProl vector has many features that make it an efficient tool for Agrobacterium-mediated transformation, and most importantly, its design minimizes the potential impact the promoter/selectable marker gene cassette has on the fidelity of the expression controlled by the test promoter.
ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome
- James G. Thomson, Yuan-Yeu Yau, R. Blanvillain, D. Chiniquy, R. Thilmony, D. Ow
- BiologyTransgenic Research
- 1 April 2009
The precise site-specific deletion by the ParA recombination system in planta demonstrates that the Para recombinase can be used to remove transgenic DNA, such as selectable markers or other introduced transgenes that are no longer desired in the final product.
PMR5, an acetylation protein at the intersection of pectin biosynthesis and defense against fungal pathogens.
- D. Chiniquy, W. Underwood, S. Somerville
- BiologyThe Plant journal : for cell and molecular…
- 12 October 2019
It is shown that heterologously expressed PMR5 protein transfers acetyl groups from [14 C]- acetyl-CoA to oligogalacturonides through site-directed mutagenesis, which illustrates that cell wall composition is intimately connected to fungal disease resistance, and outline a potential route for engineering powdery mildew resistance into susceptible crop species.
Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics
It is demonstrated that iron homeostasis within the root is impacted by drought stress, and that loss of a plant phytosiderophore iron transporter impacts microbial community composition, leading to significant increases in the drought-enriched lineage, Actinobacteria.
Metabolomics of sorghum roots during nitrogen stress reveals compromised metabolic capacity for salicylic acid biosynthesis
It is suggested that interactions between biotic and abiotic stresses may affect metabolic capacity for plant defense and need to be concurrently prioritized as breeding programs become established for biofuels production on marginal soils.
Cell Wall Composition and Candidate Biosynthesis Gene Expression During Rice Development.
An extensive analysis of cell wall composition throughout rice development, identifies genes likely to synthesize grass cell walls, and provides a framework for development of genetically improved grasses for use in lignocellulosic biofuel production and agriculture.