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Synthesis and properties of oligodeoxyribonucleotide-polyethylene glycol conjugates.
Pools of oligonucleotide conjugates consisting of 10-400 different molecular species were synthesized. The conjugates contained a varying number of ethylene glycol units attached to 3'-terminal,Expand
Oligonucleotide duplexes containing CC(A/T)GG stimulate cleavage of refractory DNA by restriction endonuclease EcoRII
Some DNA species are resistant towards the restriction endonuclease EcoRII despite the presence of unmodified recognition sites. We show that 14 base‐pair oligonucleotide duplexes containing theExpand
Hybridization-based affinity partitioning of nucleic acids using PEG-coupled oligonucleotides.
Polyethylene glycol (PEG)-coupled oligonucleotides are partitioned in an aqueous two-phase system PEG/dextran. The affinity of the oligonucleotide for the PEG-rich phase increases proportionally toExpand
Chemical synthesis of 2'-deoxyoligonucleotides containing 5-fluoro-2'-deoxycytidine.
2'-Deoxyoligonucleotides with 5-fluorocytosine residues incorporated at specific positions of the nucleotide sequence are tools of great potential in the study of the catalytic mechanism by which DNAExpand
Covalent Attachment of Methylene Blue to Oligonucleotides
Abstract The synthesis and application of oligonucleotides derivatized by methylene blue are described. For that, a carboxylated methylene blue derivative was synthesized and transformed into anExpand
A New Approach to the Synthesis of 2-Aminopurine-2′-deoxyriboside via Tri-n-butyltin Hydride Reduction
Abstract A simple conversion of guanosine to 2-aminopurine-2′-deoxyriboside is described. Following the synthesis of the 2′-phenoxythiocarbonyl derivative of 6-chloroguanosine,Expand
Chromatographic fractionation of nucleic acids using microcapsules made from plant cells
Abstract The chromatographic fractionation of nucleic acids and oligodeoxyribonucleotides on a vesicular packing (VP) material has been investigated. As the VP material consists of microcapsules withExpand
Synthesis of substituted 5-fluoro-5,6-dihydropyrimidines.
The reaction of 5-substituted uracils with fluorine in acetic acid and other solvents and the following treatment with different alcohols yielded the correspondingExpand
Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. VI. The binding and cleavage of substrates containing nucleotide analogs.
The present study deals with the binding and cleavage by EcoRII endonuclease of concatemer DNA duplexes containing EcoRII recognition sites (formula; see text) in which dT is replaced by dU orExpand