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Sphingosine‐1‐phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration
TLDR
It is demonstrated that S1P, produced in the mitochondria mainly by sphin‐gosine kinase 2 (SphK2), binds with high affinity and specificity to prohibitin 2 (PHB2), a highly conserved protein that regulates mitochondrial assembly and function.
Plasma Levels of Apoliporptoein A1 in Malaria-Exposed Primigravidae Are Associated with Severe Anemia
TLDR
Levels of the lipid metabolism protein Apolipoprotein (Apo)-AI are significantly lower in severely anemic primigravidae with PM, and ApoA1 levels positively correlate with hemoglobin levels in primig Gravid but not multigravid women.
Combining capillary electrophoresis with mass spectrometry for applications in proteomics
TLDR
This review attempts to elucidate how the most promising CE modes, capillary zone electrophoresis (CZE) and capillary isoelectric focusing (CIEF), might best be applied to MS‐based proteomics.
Using size exclusion chromatography‐RPLC and RPLC‐CIEF as two‐dimensional separation strategies for protein profiling
TLDR
There were significant differences in the sets of proteins detected and both approaches provide a basis for accurately quantifying changes in protein and modified protein abundances.
A reevaluation of computed proton affinities for the common α-amino acids
Proteomic profiling of intact proteins using WAX-RPLC 2-D separations and FTICR mass spectrometry.
TLDR
The combination of weak anion exchange fractionation and on-line reversed-phase liquid chromatography (RPLC) separation using a 12 T FTICR mass spectrometer for the detection of intact proteins from a Shewanella oneidensis MR-1 cell lysate provides a platform for high-throughput selection of biologically relevant targets for further characterization.
The reactivity of human serum albumin toward trans-4-hydroxy-2-nonenal.
TLDR
Although the kinetic data indicate that the lysines and histidines can react at relatively similar rates, the results show that lysine addition is much less favorable thermodynamically; this suggests that under physiological conditions, HNE addition to l Lysine is only relevant in situations where unusually high HNE concentrations or access to irreversible secondary reactions are found.
Paraquat exposure and Sod2 knockdown have dissimilar impacts on the Drosophila melanogaster carbonylated protein proteome
TLDR
By both Western blotting and proteomics, Paraquat exposure, but not Sod2 knockdown, resulted in increased carbonylated protein relative abundance, in good agreement with that obtained using a commercial biotin quantitation kit.
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