• Publications
  • Influence
Sequence variation of the hydroxymethyldihydropterin pyrophosphokinase: dihydropteroate synthase gene in lines of the human malaria parasite, Plasmodium falciparum, with differing resistance to
TLDR
To investigate a possible genetic basis for clinical resistance to sulfa drugs, the complete H2Pte synthase domains from eleven isolates of P. falciparum with diverse geographical origins and levels of sulfadoxine resistance were sequenced. Expand
A mutation-specific PCR system to detect sequence variation in the dihydropteroate synthetase gene of Plasmodium falciparum.
TLDR
PCR diagnostic assays based on allele-specific amplification that can reliably discriminate all of the mutations observed to alter codons 436, 581 and 613 should prove invaluable in further assessing the contribution of specific base changes in the DHPS gene of the parasite to the sulphadoxine resistance phenotype and to the clinical failure of the sulphADoxine/pyrimethamine combination Fansidar. Expand
The Multiple cpb Cysteine Proteinase Genes ofLeishmania mexicana Encode Isoenzymes That Differ in Their Stage Regulation and Substrate Preferences*
TLDR
The results suggest that the individual isoenzymes have distinct roles in the parasite’s interaction with its host, and reflects the adaptation of cathepsin L-like cysteine proteinases to diverse functions in parasitic protozoa. Expand
Processing and trafficking of cysteine proteases in Leishmania mexicana.
TLDR
Results show that auto-activation is not required for correct processing of CPB in vivo, and reveal that CPA can directly or indirectly process CPB(C25G) and suggest that cysteine proteases are targeted to lysosomes via the flagellar pocket. Expand
Gastrointestinal helminths of pipistrelle bats (Pipistrellus pipistrellus/Pipistrellus pygmaeus) (Chiroptera: Vespertilionidae) of England
TLDR
It was shown that male bats exhibited a significantly more aggregated helminth distribution and lower abundance than females, and a revised phylogeny of bat-associated Lecithodendriidae, incorporating novel L. spathulatum and Prosthodendrium sp. Expand
Toxoplasma gondii: prevalence in species and genotypes of British bats (Pipistrellus pipistrellus and P. pygmaeus).
TLDR
Using a collection of dead/euthanased bats collected from Lancashire, UK, two species of bats were tested using a highly sensitive SAG1-PCR method and the locally large and panmictic gene pool suggests that intraspecies transmission could be applicable. Expand
An Essential Role in Molting and Morphogenesis of Caenorhabditis elegans for ACN-1, a Novel Member of the Angiotensin-converting Enzyme Family That Lacks a Metallopeptidase Active Site*
TLDR
It is proposed that alteration of the ancestral ACE during evolution, by loss of the metallopeptidase active site and the addition of new protein modules, has provided opportunities for novel molecular interactions important for post-embryonic development in nematodes. Expand
The Caenorhabditis elegans Orthologue of Mammalian Puromycin-sensitive Aminopeptidase Has Roles in Embryogenesis and Reproduction*
TLDR
It is concluded that pam-1 encodes an aminopeptidase that clusters phylogenetically with the PSAs, despite attenuated sensitivity toward puromycin, and that it functions in embryo development and reproduction of the nematode. Expand
Roles of cysteine proteinases of trypanosomes and Leishmania in host-parasite interactions.
TLDR
Analysis of parasites rendered defective in cysteine proteinase function, either through genetic manipulation or through the use of specific inhibitors, has revealed roles for the enzymes in parasite virulence, in modulation of the host's immune response and in parasite differentiation. Expand
The Stage-regulated Expression of Leishmania mexicanaCPB Cysteine Proteases Is Mediated by an Intercistronic Sequence Element*
TLDR
It is proposed that the InS mediates metacyclic-specific stage-regulated expression of Leishmania mexicana by affecting the maturation of polycistronic pre-mRNA. Expand
...
1
2
3
...