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The International Committee on Taxonomy of Viruses
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Virus Taxonomy
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Rift Valley fever virus L segment: correction of the sequence and possible functional role of newly identified regions conserved in RNA-dependent polymerases.
TLDR
Using the recently released coordinates of human immunodeficiency virus reverse transcriptase and the alignment between all RNA-dependent polymerases in the 'polymerase module', the position of the conserved residues in these polymerases is determined and their possible functions are discussed.
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Development of a competitive ELISA for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleoprotein.
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Mutagen-directed attenuation of Rift Valley fever virus as a method for vaccine development.
Serial mutagenesis with 5-fluorouracil has been employed to derive an attenuated strain of Rift Valley fever virus for use as a live virus vaccine.
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Sequences and coding strategies of the S RNAs of Toscana and Rift Valley fever viruses compared to those of Punta Toro, Sicilian Sandfly fever, and Uukuniemi viruses.
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Sequencing studies of pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA
TLDR
The term ambisense RNA is coined to describe this novel coding strategy of a viral RNA that allows the two subgenomic mRNA species to be regulated independently from each other or from other viral mRNA species.
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Structural proteins of La Crosse virus
TLDR
Electron microscopy of purified La Crosse virus indicated that the virus particle is enveloped and possesses irregular surface projections (length, 10 nm) and the approximate number of protein molecules per virion has been determined.
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Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins.
TLDR
The requirements for high level expression of three foreign proteins using the polyhedrin gene promoter of Autographa californica nuclear polyhedrosis virus (AcNPV, Baculoviridae) have been investigated and it has been estimated that LCMV N protein represented approximately 50% of the total cellular protein, an observation consistent with the presence of numerous inclusion bodies in the cytoplasm of infected cells.
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Thrombospondin‐related adhesive protein (TRAP) of Plasmodium falciparum: expression during sporozoite ontogeny and binding to human hepatocytes.
TLDR
It is indicated that TRAP is not expressed in oocysts, whereas variable amounts of CS protein are found in this parasite developmental stage, and experimental evidence shows that recombinant TRAP binds to the basolateral cell membrane of hepatocytes in the Disse's space and that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.
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