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A FAM21-containing WASH complex regulates retromer-dependent sorting.
It is demonstrated that WASH associates with tubulin and localizes to early endosomal subdomains, which are enriched in Arp2/3, F-actin, and retromer components, supporting a model wherein WASH links retroming-mediated cargo containing tubules to microtubules for Golgi-directed trafficking and generates F-Actin-driven force for tubule scission. Expand
Structure and Control of the Actin Regulatory WAVE Complex
The structure shows that the activity-bearing VCA motif of WAVE is sequestered by a combination of intramolecular and intermolecular contacts within the WRC, and Rac and kinases appear to destabilize a WRC element that is necessary for VCA sequestration, suggesting the way in which these signals stimulate WRC activity towards the Arp2/3 complex. Expand
VAV proteins as signal integrators for multi-subunit immune-recognition receptors
This review focuses on the role of VAV proteins in the regulation of lymphocyte development and function, and emphasizes the regulatory roles that these proteins have through both GEF-dependent and -independent mechanisms. Expand
Inhibition of glycogen synthase kinase-3.
Two methods of GSK-3 inhibition are discussed, which can be used to determine the involvement of G SKS-3 in a cellular process of interest. Expand
NKG2D-DAP10 triggers human NK cell–mediated killing via a Syk-independent regulatory pathway
It is shown that a crucial Tyr-Ile-Asn-Met amino acid motif in the cytoplasmic tail of DAP10 couples receptor stimulation to the downstream activation of phosphatidylinositol 3-kinase, Vav1, Rho family GTPases and phospholipase C. Expand
Regulation of T-cell activation by the cytoskeleton
Recent advances that have furthered the understanding of the crucial importance of the T-cell cytoskeleton in controlling these aspects of T- cell immune recognition are discussed. Expand
Formins regulate the actin-related protein 2/3 complex-independent polarization of the centrosome to the immunological synapse.
Without Arp2/3-mediated actin nucleation, stimulated T cells could not form an F-actin-rich lamellipod, but instead produced polarized filopodia-like structures, and formins are essential cytoskeletal regulators of centrosome polarity in T cells. Expand
NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells
It is shown here that binding of the p85 subunit of phosphatidylinositol-3- kinase to DAP10 could not by itself trigger cell-mediated cytotoxicity and thatbinding of an intermediate consisting of the DAP 10 binding partner Grb2 and the effector molecule Vav1 to D AP10 was sufficient to initiate tyrosine-phosphorylation events. Expand
WASH and WAVE actin regulators of the Wiskott–Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes
It is shown that WASH functions in cells within a 500 kDa core complex containing Strumpellin, FAM21, KIAA1033 (SWIP), and CCDC53, and biochemical and electron microscopic analyses show that the WASH and WAVE complexes are structurally similar. Expand
COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A
The COMMD1 protein, implicated in copper homeostasis, is found to regulate endosomal sorting of the copper transporter ATP7A through a novel protein complex containing CCDC22, CCDC93, and C16orf62,Expand