• Publications
  • Influence
The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
TLDR
It is shown that the carboxy-terminal domain of the pol II large subunit is required for efficient RNA processing, and an association between the CTD and 3′-processing factors suggests that an mRNA 'factory' exists which carries out coupled transcription, splicing and cleavage–polyadenylation of mRNA precursors. Expand
Dynamic association of capping enzymes with transcribing RNA polymerase II.
TLDR
It is shown that yeast capping enzymes cross-link in vivo to the 5' ends of transcribed genes and that this localization requires the C-terminal heptad repeat domain (CTD). Expand
Coupling mRNA processing with transcription in time and space
  • D. Bentley
  • Biology, Medicine
  • Nature Reviews Genetics
  • 1 March 2014
TLDR
Experiments using sophisticated new methods for analysis of nascent RNA have provided important insights into the relative amount of co- transcriptional and post-transcriptional processing, the relationship between mRNA elongation and processing, and the role of the Pol II carboxy-terminal domain (CTD) in regulating these processes. Expand
Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors.
  • D. Bentley
  • Biology, Medicine
  • Current opinion in cell biology
  • 1 June 2005
The universal pre-mRNA processing events of 5' end capping, splicing, and 3' end formation by cleavage/polyadenylation occur co-transcriptionally. As a result, the substrate for mRNA processingExpand
5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II.
TLDR
Results suggest that Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and capping is co-ordinated with CTD phosphorylation. Expand
TFIIH-Associated Cdk7 Kinase Functions in Phosphorylation of C-Terminal Domain Ser7 Residues, Promoter-Proximal Pausing, and Termination by RNA Polymerase II
TLDR
It is shown that both Cdk7 and Cdk9/PTEFb contribute to phosphorylation of Pol II CTD Ser5 residues on transcribed genes, suggesting a new role for TFIIH at 3′ ends. Expand
The role of Rat1 in coupling mRNA 3'-end processing to transcription termination: implications for a unified allosteric-torpedo model.
TLDR
It is reported that two exonucleases, Rat1 and Xrn1, both contribute to cotranscriptional degradation of nascent RNA, but this degradation is not sufficient to cause polymerase release. Expand
Functional interaction of yeast pre-mRNA 3' end processing factors with RNA polymerase II.
The RNA polymerase II CTD is essential for 3' end cleavage of metazoan pre-mRNAs and binds 3' end processing factors in vitro. We show genetic and biochemical interactions between the CTD and theExpand
"Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions.
TLDR
RNA maturation factors are not the only nuclear machines whose work is organized cotranscriptionally around the TEC scaffold, and TECs can be viewed as potent "community organizers" within the nucleus. Expand
Pre-mRNA splicing is facilitated by an optimal RNA polymerase II elongation rate.
TLDR
It is suggested that an optimal rate of transcriptional elongation is required for normal cotranscriptional pre-mRNA splicing. Expand
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