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Functional metaproteome analysis of protein extracts from contaminated soil and groundwater
An extraction method in which the separation of proteins from the inorganic and organic constituents of the soil matrix was achieved by a combination of 0.1 M NaOH treatment and phenol extraction enabled the analysis of the metaproteome of soil and groundwater samples and provides a means to study the diversity of environmental microbial communities while addressing functional aspects more directly than metagenome or even metatranscriptome analysis.
Insights into Pseudomonas putida KT2440 response to phenol‐induced stress by quantitative proteomics
The information emerging from this genome expression profiling and the detailed investigation of the biological role of candidate genes, as targets of phenol toxicity or as determinants of Phenol resistance in P. putida KT2440, will allow more rationale strategies for developing bacteria with greater solvent tolerance with impact in bioremediation and whole‐cell biotransformations in media with organic solvents.
The Alkyl tert-Butyl Ether Intermediate 2-Hydroxyisobutyrate Is Degraded via a Novel Cobalamin-Dependent Mutase Pathway
It is demonstrated for the newly isolated β-proteobacterial strains L108 and L10, as well as for the closely related strain CIP I-2052, that 2-HIBA was degraded by a cobalamin-dependent enzymatic step.
Advanced tool for characterization of microbial cultures by combining cytomics and proteomics
This study combined flow cytometry and gel-free proteomic analysis to investigate an artificial mixed bacterial culture consisting of Escherichia coli K-12 and Pseudomonas putida KT2440, and investigated the physicochemical properties, namely Mr, pI, hydropathicity, and transmembrane helices of the proteins covered.
Continuous Synthesis and Excretion of the Compatible Solute Ectoine by a Transgenic, Nonhalophilic Bacterium
In order to synthesize ectoine continuously in less-aggressive media, the ectoine genes ectABC of the halophilic bacterium Chromohalobacter salexigens are introduced into an Escherichia coli strain using the expression vector pASK-IBA7.
Vitellogenin cleavage products as indicators for toxic stress in zebra fish embryos: A proteomic approach
Vitellogenins (Vtgs) are the major yolk proteins in all oviparous animals. Systematic and regulated processing of these during embryogenesis is crucial for embryonic development. In the present
Metagenome and metaproteome analyses of microbial communities in mesophilic biogas-producing anaerobic batch fermentations indicate concerted plant carbohydrate degradation.
Microbial communities in biogas batch fermentations, using straw and hay as co-substrates, were analyzed at the gene and protein level by metagenomic and metaproteomic approaches and it was found that key enzymes of methanogenesis were expressed in high quantities, indicating the high metabolic activity of meethanogens, although they only represented a minor group within the microbial community.
Response of Pseudomonas putida KT2440 to phenol at the level of membrane proteome.
Results suggest the important role of protein chaperones, of cell envelope and cell surface and of a more active respiratory chain in the response to phenol, and mechanistic insights may be extended to Pseudomonas adaptation to solvents, of possible impact in biodegradation, bioremediation and biocatalysis.
Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress.
Some special proteins were induced by phenol or catechol, which might be useful as biomarkers for chemostress, and could be involved in the catalytic degradation of potentially toxic compounds.
Improving protein extraction and separation methods for investigating the metaproteome of anaerobic benzene communities within sediments
The identification of several proteins confirmed that this procedure has the capacity to analyse the metaproteome of an anoxic living microbial community.