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The active site of cellobiohydrolase Cel6A from Trichoderma reesei: the roles of aspartic acids D221 and D175.
- A. Koivula, L. Ruohonen, +13 authors T. A. Jones
- Chemistry, Medicine
- Journal of the American Chemical Society
- 2 August 2002
Trichoderma reesei cellobiohydrolase Cel6A is an inverting glycosidase. Structural studies have established that the tunnel-shaped active site of Cel6A contains two aspartic acids, D221 and D175,… Expand
Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant.
The crystal structures of Family 7 glycohydrolases suggest that a histidine residue near the acid/base catalyst could account for the higher pH optimum of the Humicola insolens endoglucanase Cel7B,… Expand
Blausäure‐N‐methylid: Darstellung, spektroskopische Eigenschaften und seine Beziehung zu anderen C2H3N‐Isomeren
Hydrogen Cyanide N-Methylide: Preparation, Spectroscopic Identification, and Its Relationship to Other C2H3N Isomers With the intention of isolating 1H-azirene (8) we studied several… Expand
A novel synthesis of protected glucose intermediates
The 1,2,4,6-Tetra-O-pivaloyl-D-gluco-pyranose 3 and 1,2,3,6-Tetra-O-pivaloyl-D-gluco-pyranose 4 have been prepared in one step from anhydrous glucose and pivaloyl chloride. These new intermediates… Expand
Hydrolyses of α-and β-cellobiosyl fluorides by Cel 6 A ( cellobiohydrolase II ) of Trichoderma reesei and Humicola insolens
Wehave measured the hydrolyses of αand β-cellobiosyl fluorides by theCel6A [cellobiohydrolase II (CBHII)] enzymes ofHumicola insolens and Trichoderma reesei, which have essentially identical crystal… Expand
About the Stereochemistry of Intramolecular (2 + 2) Photocycloadditions.
DEOXYSCHIZANDRIN, STEREOCHEMISTRY AND TOTAL SYNTHESIS
United States Patent ( 19 ) Becker ( 54 ) PROCESS FOR THE PURIFICATION OF SEWAGE WHILE RECAPTURING THE FATTY AND ALBUMINOUS MATTER IN REUSABLE FORM 76 ) Inventor :
- D. Becker
3,000,790 9/1961 Jeanes ................................ 210/54 R. 3,082,173 3/1963 Horvitz .................................. 210/52 3,236,766 2/1966 Levin ..... ... 210/44 3,248,225 4/1966 Bode… Expand
Ring Opening of 3-Methoxyacenaphthylene-1,2-dione. Application of13C- 2D-Inadequate-NMR Spectroscopy to Structure Assignment of Some Substituted Naphthalenes.
A Novel Synthesis of Protected Glucose Intermediates.