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An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.
In Escherichia coli, RNA degradation often begins with conversion of the 5'-terminal triphosphate to a monophosphate, creating a better substrate for internal cleavage by RNase E. Remarkably, noExpand
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Properties of a Bacillus subtilis polynucleotide phosphorylase deletion strain.
The pnpA gene of Bacillus subtilis, which codes for polynucleotide phosphorylase (PNPase), has been cloned and employed in the construction of pnpA deletion mutants. Growth defects of both B.Expand
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Bacillus subtilis polynucleotide phosphorylase 3′-to-5′ DNase activity is involved in DNA repair
In the presence of Mn2+, an activity in a preparation of purified Bacillus subtilis RecN degrades single-stranded (ss) DNA with a 3′ → 5′ polarity. This activity is not associated with RecN itself,Expand
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Induced mRNA stability in Bacillus subtilis.
We have investigated the induced stability of mRNA encoded by the ermC gene in Bacillus subtilis. Induction of ermC gene expression by erythromycin is known to occur at the translational level. WeExpand
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Effect of 5′‐proximal elements on decay of a model mRNA in Bacillus subtilis
Previous work showed that a 42‐nucleotide sequence from an SP82 bacteriophage early RNA functions as a 5′ mRNA stabilizer in Bacillus subtilis. Real‐time reverse transcriptase polymerase chainExpand
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A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake
The atp operon of alkaliphilic Bacillus pseudofirmus OF4, as in most prokaryotes, contains the eight structural genes for the F-ATPase (ATP synthase), which are preceded by an atpI gene that encodesExpand
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Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells.
Cloning of specific regions of RK2, a broad host range incompatibility group P plasmid, has revealed three genes: kilA, kilB, and kilC. Each of these genes can cause loss of viability of anExpand
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Replication control genes of plasmid pE194.
pE194, a 3.7-kilobase plasmid, confers resistance to macrolide, lincosamide, and streptogramin B antibiotics. The previously identified cop and repF genes of pE194 have been further localized byExpand
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Chromosomal tetA(L) gene of Bacillus subtilis: regulation of expression and physiology of a tetA(L) deletion strain.
Deletion of the tetA(L) chromosomal region of Bacillus subtilis in a strain designated JC112 increased the strain's sensitivity to low tetracycline concentrations. It also resulted in phenotypicExpand
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Recycling of a regulatory protein by degradation of the RNA to which it binds.
When Bacillus subtilis is grown in the presence of excess tryptophan, transcription of the trp operon is regulated by binding of tryptophan-activated TRAP to trp leader RNA, which promotesExpand
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