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An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.
TLDR
A system based on co-expression of a viral-encoded suppressor of gene silencing, the p19 protein of tomato bushy stunt virus, that prevents the onset of PTGS in the infiltrated tissues and allows high level of transient expression is described. Expand
A species of small antisense RNA in posttranscriptional gene silencing in plants.
TLDR
The 25-nucleotide antisense RNA detected in transgene-induced PTGS is likely synthesized from an RNA template and may represent the specificity determinant of PTGS. Expand
RNA silencing in plants
TLDR
Diverse biological roles of these pathways have been established, including defence against viruses, regulation of gene expression and the condensation of chromatin into heterochromatin, and the full extent of this functional diversity in genetic and epigenetic mechanisms of genome control is investigated. Expand
Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants.
TLDR
Results suggest that virus-encoded suppressors of gene silencing have distinct modes of action, are targeted against distinct components of the host gene-silencing machinery, and that there is dynamic evolution of theHost and viral components associated with the gene- silencing mechanism. Expand
Retracted: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana
TLDR
By expressing PVY and CMV‐encoded proteins in a PVX vector, it is shown that the viral suppressors of gene silencing are the HCPro of PVy and the 2b protein of CMV. Expand
Technical Advance. Tobacco rattle virus as a vector for analysis of gene function by silencing.
TLDR
An infectious cDNA clone of tobacco rattle virus that has been modified to facilitate insertion of non-viral sequence and subsequent infection to plants is described and it is shown that this vector mediates VIGS of endogenous genes in the absence of virus-induced symptoms. Expand
Criteria for Annotation of Plant MicroRNAs
TLDR
The specific criteria required for the annotation of plant miRNAs are updated, including experimental and computational data, as well as refinements to standard nomenclature. Expand
Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs.
TLDR
It is shown that AGO1 selectively recruits certain classes of short silencing-related RNA, and it is predicted that other Arabidopsis AGOs might have a similar catalytic activity but recruit different subsets of siRNAs or miRNAs. Expand
Two classes of short interfering RNA in RNA silencing
TLDR
It is shown that siRNAs produced in plants from a green fluorescent protein (GFP) transgene are in short and long size classes, whereas those from endogenous retroelements are only in the long class, revealing an unexpected level of complexity in the RNA silencing pathway in plants that may also apply in animals. Expand
RNA Polymerase IV Directs Silencing of Endogenous DNA
TLDR
By mutation of the two largest subunits (NRPD1a and NRPD2), it is shown that Pol IV silences certain transposons and repetitive DNA in a short interfering RNA pathway involving RNA-dependent RNA polymerase 2 and Dicer-like 3. Expand
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