Capacity for methane oxidation in landfill cover soils measured in laboratory-scale soil microcosms
- D. Kightley, D. B. Nedwell, M. Cooper
- Medicine, ChemistryApplied and Environmental Microbiology
- 1 February 1995
Laboratory-scale soil microcosms containing different soils were permeated with CH(inf4) for up to 6 months to investigate their capacity to develop a methanotrophic community to attribute this to a facultative, possibly mixotrophic, methanOTrophic microbial community.
Diversity and Abundance of Nitrate Reductase Genes (narG and napA), Nitrite Reductase Genes (nirS and nrfA), and Their Transcripts in Estuarine Sediments
- Cindy J. Smith, D. B. Nedwell, Liang-fei Dong, A. Osborn
- Environmental ScienceApplied and Environmental Microbiology
- 30 March 2007
Analysis of cloned PCR-amplified narG, napA, and nrfA gene sequences showed the indigenous nitrate-reducing communities to be both phylogenetically diverse and also divergent from previously characterized nitrate reduction sequences in soils and offshore marine sediments and from cultured nitrate reducers.
Environmental costs of freshwater eutrophication in England and Wales.
- J. Pretty, C. Mason, D. B. Nedwell, R. Hine, S. Leaf, R. Dils
- Environmental Science, EconomicsEnvironmental Science and Technology
- 15 January 2003
A new framework of cost categories that assess both social and ecological damage costs and policy response costs is developed, indicating the severe effects of nutrient enrichment and eutrophication on many sectors of the economy.
Dissimilatory reduction of nitrate to ammonium, not denitrification or anammox, dominates benthic nitrate reduction in tropical estuaries
- Liang-fei Dong, M. Sobey, D. B. Nedwell
- Environmental Science
- 1 January 2011
We measured benthic denitrification (DN) and dissimilatory reduction of nitrate to ammonium (DNRA) using the isotope‐pairing technique in three tropical estuaries in Thailand (Mae Klong), Indonesia…
Changes in Benthic Denitrification, Nitrate Ammonification, and Anammox Process Rates and Nitrate and Nitrite Reductase Gene Abundances along an Estuarine Nutrient Gradient (the Colne Estuary, United…
- Liang-fei Dong, Cindy J. Smith, S. Papaspyrou, A. Stott, A. Osborn, D. B. Nedwell
- Environmental ScienceApplied and Environmental Microbiology
- 20 March 2009
Numbers of narG genes declined along the estuary, while napA gene numbers were stable, suggesting that NAP-mediated nitrate reduction remained important at low nitrate concentrations, and log-log relationships indicate an underlying relationship between the genetic potential for nitrate Reduction and the corresponding process activity.
Identification of active methylotroph populations in an acidic forest soil by stable-isotope probing.
- S. Radajewski, G. Webster, J. Murrell
- BiologyMicrobiology
- 1 August 2002
Results from the (13]CH(3)OH and (13)CH(4) SIP experiments provide a rational basis for further investigations into the ecology of methylotrophic carbon metabolism, and suggest moderately acidophilic methylotroph populations were active in the microcosms.
Evaluation of quantitative polymerase chain reaction-based approaches for determining gene copy and gene transcript numbers in environmental samples.
- Cindy J. Smith, D. B. Nedwell, Liang-fei Dong, A. Osborn
- Biology, MedicineEnvironmental Microbiology
- 1 May 2006
Q-PCR determination of absolute numbers of genes and transcripts using environmental nucleic acids should be treated cautiously because of the high variability in gene and transcript number variability.
Detection and Enumeration of Sulphate-Reducing Bacteria in Estuarine Sediments by Competitive PCR
- R. Kondo, D. B. Nedwell, K. Purdy, S. Silva
- Biology
- 1 April 2004
The results show that the newly developed competitive PCR technique targeted to dsr is a powerful tool for rapid and reproducible estimation of SRB numbers in situ and is superior to the use of culture-dependent techniques.
Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh
- M. Munson, D. B. Nedwell, T. Embley
- BiologyApplied and Environmental Microbiology
- 1 December 1997
Recovery of sequences closely related to those of methanogen such as Methanococcoides and Methanolobus, which can use substrates other than hydrogen, provides support for published hypotheses that such methanogens are probably important in sulfate-rich sediments and identifies some likely candidates.
Isolation of haloarchaea that grow at low salinities.
- K. Purdy, T. Cresswell-Maynard, T. Embley
- BiologyEnvironmental Microbiology
- 1 June 2004
The existence of Archaea that can grow in non-extreme conditions and of a diverse community of haloarchaea existing in coastal salt marsh sediments are revealed, suggesting that the ecological range of these physiologically versatile prokaryotes is much wider than previously supposed.
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