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Ultra-wideband (UWB) electromagnetic pulses of nanosecond duration, or nanopulses, are of considerable interest to the communications industry and are being explored for various applications in biotechnology and medicine. The propagation of a nanopulse through biological matter has been computed using the finite difference-time domain (FDTD) method. The(More)
We demonstrate a method for obtaining accurate estimates of the thermodynamic parameter values characteristic of a two-state folding/unfolding transition under conditions in which the onset of cold denaturation prevents the native state from being fully populated at any temperature. This situation occurs for proteins exhibiting low thermal stability, which(More)
Four peptides encompassing the entire amino acid sequence of hen lysozyme were examined in aqueous solution and in 50% (v/v) 2,2,2-trifluoroethanol (TFE) by far-UV CD. Two peptides, 1-40 and 84-129, correspond to regions which are helical in the native protein, and together represent the alpha-domain. The beta-domain of the native enzyme was also(More)
The cooperative nature of the protein folding process is independent of the characteristic fold and the specific secondary structure attributes of a globular protein. A general folding/unfolding model should, therefore, be based upon structural features that transcend the peculiarities of alpha-helices, beta-sheets, and other structural motifs found in(More)
Fusion proteins are used to enhance the yields of heterologous proteins secreted from filamentous fungi. In Aspergillus niger, the target protein is normally fused downstream of the carrier protein glucoamylase with a Lys-Arg KEX2-like cleavage site at the junction. This is cleaved in vivo to release mature protein but the processing is not always accurate.(More)
Conducting nanowires have been found to self-assemble between microelectrodes from palladium acetate solution on applying an alternating electric field of relatively high intensity and frequency. The wires grew spontaneously along the direction of the electric field and have high uniformity and conductivity. The finding challenges current metallic nanowire(More)
Hydrogen exchange measurements on equine lysozyme show that amides in three of the four major helices of the native protein are significantly protected in a molten globule state formed at pH 2. The pattern of protection within the different helices, however, varies significantly. Examination of the pattern in the light of the native structure indicates that(More)
Tensin, an actin filament capping protein, and auxilin, a component of receptor-mediated endocytosis, are known to have 350 residue regions of significant sequence similarity near their N-termini (Schröder et al., 1995, Eur J Biochem 228:297-304). Here we demonstrate that these regions are homologous, not only to each other, but also to the catalytic domain(More)