D Strahilov

  • Citations Per Year
Learn More
Antiserum to the epimeraseless Rc mutant of Salmonella minnesota was prepared by immunization of rabbits. The antiserum provided good protection in mice i.p. challenged with 125 and 25 LD50 doses of live heterologous Gram-negative bacteria i. e. S. typhimurium, Escherichia coli and Pseudomonas aeruginosa. The protection against Klebsiella pneumoniae was(More)
Various gram-negative bacteria were subjected to mild acid hydrolysis. The acid-treated bacteria exhibited strong cross-reactivity with fluorescein isothiocyanate-conjugated antiserum to the Re mutant of Salmonella minnesota. Hydrolyzed bacteria showed considerably stronger fluorescence than heat-treated bacteria. It is assumed that acid hydrolysis uncovers(More)
Lipopolysaccharides (LPS) derived from a single Salmonella serotype or a combination of serotypes were submitted to insolubilization by means of glutaraldehyde. Cross-linked LPS proved to be efficient immunoadsorbents. They were employed for the removal of cross-reacting anti-O antibodies from various Salmonella antisera. LPS immunoadsorbents offer a number(More)
Four murine hybridoma clones secreting monoclonal antibodies (mAb) directed against lipopolysaccharide (LPS) antigen of S. newington, serogroup E1, were obtained after a fusion of spleen cells of mice immunized with formaldehyde-killed bacteria and mouse myeloma cells of the X63-Ag8.653 line. Antigen binding properties and specificity of the mAb were(More)
  • 1