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Ribosome-inactivating proteins (RIPs) are a family of enzymes that trigger the catalytic inactivation of ribosomes. The most known member of the family is the highly poisonous two-chain ricin isolated from Ricinus communis L. Sambucus species contain a number of two-chain RIPs structurally and enzymatically related to ricin which have the noteworthy feature(More)
This paper describes the design, development and field evaluation of a machine translation system from Spanish to Spanish Sign Language (LSE: Lengua de Signos Española). The developed system focuses on helping Deaf people when they want to renew their Driver’s License. The system is made up of a speech recognizer (for decoding the spoken utterance into a(More)
Keywords: Spanish sign language (LSE) Speech generation from LSE LSE corpus Sign editor LSE translation Driver's license renewal a b s t r a c t This paper describes the development of a Spoken Spanish generator from sign-writing. The sign language considered was the Spanish sign language (LSE: Lengua de Signos Española). This system consists of an advanced(More)
Pathophysiological processes associated with oxidative stress lead to the generation of reactive lipid species. Among them, lipids bearing unsaturated aldehyde or ketone moieties can form covalent adducts with cysteine residues and modulate protein function. Through proteomic techniques we have identified actin as a target for the addition of biotinylated(More)
Cyclic guanosine monophosphate (cGMP) has been recently shown to modulate in vitro capacitation of mammalian spermatozoa, but the mechanisms through which it influences sperm functions have not been clarified. There are at least two targets of cGMP, cyclic nucleotide-gated (CNG) channels and cGMP-dependent protein kinase (PKG), involved in several(More)
Perfluoroalkyl phosphonic acids (PFPAs), a new class of perfluoroalkyl substances used primarily in the industrial sector as surfactants, were recently detected in surface water and wastewater treatment plant effluents. Toxicological effects of PFPAs have as yet not been investigated in aquatic organisms. The objective of the present study was to evaluate(More)
A simple and mild procedure is described for the isolation of pituitary growth hormone from alpaca glands. It involves extractions in buffer at pH 8.7 and gel filtration in Sephadex G-75. an average yield of 1 mg purified product may be obtained from 1 g fresh alpaca pituitary glands. The alpaca hormone was examined for homogeneity by SDS-polyacrylamide gel(More)
The State of Florida has developed a draft radon standard for new residential construction. A study was conducted to evaluate the effectiveness of the standard's features in controlling radon entry in new houses built over relatively high radon potential soils. Special attention was given to foundation preparations and slab placements to insure that the(More)
Half-cystine peptides were isolated from the enzymatic digest of reduced and carbamidomethylated alpaca growth hormone. From their amino acid composition and determination of the end terminal residues we propose the amino acid sequence around the two disulphide bridges, partially based on homology with other mammalian growth hormones. The proposed sequence(More)