D R Straus

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The essential catalytic base at the active site of the glycolytic enzyme triosephosphate isomerase is the carboxylate group of Glu-165, which directly abstracts either the 1-pro-R proton of dihydroxyacetone phosphate or the 2-proton of (R)-glyceraldehyde 3-phosphate to yield the cis-enediol intermediate. Using the methods of site-directed mutagenesis, we(More)
The essential basic residue at the active site of the glycolytic enzyme triosephosphate isomerase is Glu-165, which is responsible for the abstraction of either the 1-pro-R proton of dihydroxyacetone phosphate or of the 2-proton of D-glyceraldehyde 3-phosphate, in the enolization steps that constitute the reaction catalysed by this enzyme. We have changed(More)
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