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The synthesis and assembly of xyloglucan were examined during early stages of wall regeneration by protoplasts isolated from growing regions of etiolated peas. During early stages of cultivation, fluorescence microscopy showed that the protoplast surface bound Calcofluor and ammonium salt of 8-anilino-1-naphthalene sulfonic acid and, in time, it also bound(More)
Soil columns were exposed to balanced (low Na+) or unbalanced (high Na+) high-salt solutions for a period of 7 days followed by 7 days of stress reflief. Total numbers of bacteria released into the perfusates rose under both types of stress, but the proportion of displaced bacteria that were viable fell significantly. Relief from both types of stress(More)
A fluorescence-staining technique using the magnesium salt of 8-anilino-1-naphthalene sulfonic acid is described and used to follow the changes in the distribution patterns of microorganisms in soils. A statistical procedure was used to determine the occurrence of significant differences in clumping of bacteria (i.e., production of colonies) in different(More)
A slide incubation chamber was described which allowed small plants to be grown from seed and the root systems to be observed microscopically. A fluorescence stain, the ammonium salt of 8-anilino-1-naphthalene sulfonic acid, was applied to the soil in which the roots were growing and the stained microorganisms on the roots and in the rhizosphere were(More)
Significant progress has been made toward commercial implementation of a large-scale somatic embryogenesis (SE) production process for spruce somatic embryos. The major emphasis was to overlay the established principles and disciplines of “Manufacturing Resource Planning” (MRP) onto the original laboratory-scale protocols used to produce spruce SE and SE(More)
Bioreactor-based plant bioprocesses are not only an excellent model for quantitative interpretation of growth kinetics and product biosynthesis, but can be a vehicle for scaled-up production of valuable bioactive natural compounds, mass propagule production, and applications using genetically engineered cells and tissues. The following series of manuscripts(More)
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