D. Liškov’a

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Cell suspension culture of poppy was permeabilized by Tween 80 and immobilized by glutaraldehyde. Β-Galactosidase showed optimum pH 4.8 and temperature of 50 ‡C. The enzyme hydrolysis was linear during 3 h reaching 68 % conversion. The cells characterized by high Β-galactosidase activity and stability in long-term storage showed convenient(More)
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