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Thermal behavior of intact and LC-2 deficient myosin obtained from bovine heart was studied using EPR and DSC techniques. The reactive thiol sites (Cys 704) of myosin was labelled with 4-maleimidopiperidine-nitroxyl, and the measurements were taken in X-band in the conventional and saturation transfer EPR time domains. DSC scans were made from 5 degrees up(More)
Activation heat (AH) is an important component in energy balance of muscle contraction. It represents energy dissipation of biochemical processes enabling muscle in rest to perform contraction. According to our measurements on intact muscles, AH is 20% of initial heat production in twitch, and 10% in tetanus. Significantly different AH values have been(More)
The dependence of the heat production of muscle on length was examined in isometric (with stretch) and isotonic (free of stretch) tetanuses of 2 s. The isometric heat production was significantly greater than the isotonic one at any length of muscle. The isometric heat coefficient (Flo/Q, where F is the active tension development of muscle measured in(More)
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