D Kadiisky

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A histochemical method for demonstration of 2',3'-cyclic nucleotide 3'-phosphohydrolase activity is proposed. Cryostat sections, fixed in chlorophorm-methanol mixture, are incubated in a solution containing cyclic adenosine 2',3'-monophosphate, acid phosphatase, lead nitrate in acetate buffer, pH = 6,4 or 6.5. This method is based on conversion by the(More)
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