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Fractionation of the highly soluble collagens released during limited pepsin digestion of whole human placenta has resulted in the isolation of a unique collagenous fraction comprised exclusively of high molecular weight aggregates. On reduction and alkylation, the aggregates dissociated yielding collagen-like polypeptides with an apparent Mr = 40,000 as(More)
A method to determine the proportions of the major fiber-forming collagens (types I, III, and V) in noncartilaginous human tissues is presented. The procedure relies on direct solubilization of tissue collagen as cyanogen bromide peptides. The peptides are subjected to cation exchange chromatography followed by gel permeation chromatography in a manner(More)
Three collagen fractions, each of which contain molecules composed of alpha 1(II) chains, have been isolated from pepsin-solubilized rat chondrosarcoma collagen. One fraction could be selectively precipitated from the pepsin digest at 0.7 M NaCl. Two additional fractions were obtained on chromatography of the collagen precipitating at 1.2 M NaCl on(More)
Monoclonal antibodies produced against pepsin-solubilized newborn rat skin type V collagen [alpha 1(V)]2 alpha 2(V), and chondrosarcoma type XI collagen [alpha 1(XI) alpha 2(XI) alpha 3(XI)] are used to localize the collagens in sections of the chondrosarcoma as well as the normal rat knee joint by indirect immunofluorescence. Immunostaining for type V(More)
Collagenous fragments from type IX molecules have been solubilized by limited pepsin proteolysis of a transplantable rat chondrosarcoma and isolated by selective salt precipitation. Chromatography of the solubilized precipitate on CM-cellulose under nondenaturing conditions yielded three fractions. When examined by polarimetry, the material in all three(More)
A gel permeation high-performance liquid chromatography system utilizing commercially-available silica-based gels has been developed for evaluation of the cyanogen bromide cleavage products derived from collagen a chains. The high efficiency and precision of the system permits unequivocal identification of various chains by inspection of the peptide elution(More)