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Possible inflexions in isothermal binding plots derived from equilibrium-dialysis measurements and in equivalent plots obtained by polarimetric measurements accord with the possibility that discrete Ca(2+)-heparin-water complexes/phases may exist, the nature and proportions of which depend on the conditions under which the interaction occurs. Analysis of(More)
Porcine and recombinant human atelocollagen I solutions were cross-linked with a water soluble carbodiimide at various stoichiometries and collagen concentrations (5-20 w/w %). The resulting hydrogels were clear and, when used as cell growth matrices, allowed cell and nerve visualization in vitro and in vivo. We have previously reported that, after six(More)
Potentiometric titrations, at ionic strengths (I) ranging from 0.0057 to 0.336, suggest that Ca2+, Cu2+, Li+ and Na+ bind to heparin in a manner that depends on cation identity. These interactions were less affected by the value of I than those of heparin with Mg2+, which binds weakly below I0.050 and with K+, which binds weakly at I0.0057. Of the(More)
Given the high degree of interoperator error and poor precision of manual platelet counting, it has recently been proposed that an immunoplatelet counting method could become the new reference procedure. Platelets are identified immunologically with a suitable monoclonal antibody, and the platelet count is derived from the ratio of fluorescent platelet(More)
By careful definition of polymer environment, heparin i.r. spectra were examined in a region (750-950 cm-1) in which sulphate half-ester absorptions occur. Changes seen in this region when metal ion-heparin complexes are converted into heparinic acid, when heparin is carboxy-group-reduced and when various concentrations of Li(+)-heparin are examined are(More)
The XE 2100 (Sysmex Corporation) is a cell counter that furthers the technology of fluorescent flow cytometry developed from the earlier range of Sysmex analysers. The new diagnostic features are a nucleated red cell count (NRBC), the ability to measure platelets by impedance as well as an 'optical' platelet count using a fluorescence dye and an immature(More)