D Girdlestone

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1. Using isolated slices of rat cingulate and sensorimotor cortex, intracellular recordings were obtained from pyramidal neurons in layer III. Simultaneous extracellular recordings were obtained from neurons in ventral layer III and layer IV. Spike-triggered averaging was employed to investigate synaptic connections from neurons in layers III/IV to(More)
Intramuscular injections of either horseradish peroxidase (HRP) or wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) were made into the masseter muscle of rats. Both tracers labeled primary sensory neurones in the V mesencephalic nucleus, motoneurones in the V motor nucleus, and some motoneurones in the facial motor nucleus. WGA-HRP labeled(More)
1. The only unifying principle of general anaesthesia is that general anaesthetics interact with membrane components and no single cellular mechanism appears to explain their widespread effects in the central nervous system. 2. The gastropod mollusc, Lymnaea stagnalis, provides an excellent model system for studies on general anaesthetics because it has(More)
1. In slices of rat neocortex, spike triggered averaging was employed to record in one neurone the excitatory postsynaptic potential (e.p.s.p.) generated by a spike in another, neighbouring neurone. When recorded at different membrane potentials, some of these e.p.s.ps exhibited a voltage relation typical of neuronal responses to N-methyl-D-aspartate(More)
1. The excitability of lumbar spinal motoneurones was studied in halothane-anaesthetized rats by recording with microelectrodes the amplitude of the population spike evoked antidromically by stimulation of the cut ventral roots. 2. Electrical stimulation of the nucleus raphe obscurus for 1 min at 20 Hz increased the population spike amplitude and, as shown(More)
1. The gastropod mollusc Lymnaea stagnalis (L.) is an ideal model system for studies on anaesthesia. It is reversibly anaesthetized by the general anaesthetics halothane, enflurane and isoflurane. 2. Criteria for "anaesthesia" in Lymnaea were established. The reflex used in ED50 trials was the whole animal withdrawal reflex. 3. ED50 values for halothane,(More)
1. RP 62203 (2-[3-(4-(4-fluorophenyl)-piperazinyl)propyl]naphto[1,8- ca]isothiazole-1,1-dioxide) is a novel naphtosultam derivative which shows very high affinity for 5-HT2 receptors in the rat cerebral cortex (Ki = 50.0 pM). 2. RP 62203 is relatively selective for this sub-type of 5-hydroxytryptamine (5-HT) receptor, having lower affinity for the 5-HT1A(More)
In order to clarify the participation of substance P in the expression of opiate withdrawal, we have investigated the effects induced by the new selective neurokinin-1 antagonist RP 67580 on naloxone-induced morphine withdrawal syndrome in rats. Intracerebroventricular administration of RP 67580 elicited a decrease in 7 of the 13 withdrawal signs evaluated.(More)
Snails exposed to the general anaesthetic halothane show an increase in biting plus mouthing movements. Perfusion of the isolated CNS with halothane leads to a period of increased spiking activity, followed by suppression of activity in identified feeding motoneurones in the buccal ganglia. Synaptic inputs to motoneurones from interneurones of the buccal(More)