D F Kiszkiss

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A denitrifying mutant of Bacillus stearothermophilus NCA 2184, strain 2184-D, was used to explore the development of nitrate respiration in relation to oxygen respiration. Aerobically grown wild-type cultures could acquire the ability to use nitrate as a result of selection of nitrate-respiring mutants by the presence of nitrate and a reduced oxygen(More)
Study of the numbers of thymus-derived lymphocytes by the rosette assay (T-RFC) in patients with leprosy reveals that lower than normal numbers of T-RFC are regularly seen in those patients with the active lepromatous form of this disease. Essentially normal numbers of T-RFC were found in inactive lepromatous, borderline, and indeterminate types of leprosy.(More)
Membranes were isolated from Bacillus stearothermophilus 2184D by lysozyme digestion of the cell wall and subsequent differential centrifugation. Observations with the electron microscope indicate that such membranes are relatively intact and have a typical membrane appearance. Nitrate will preferentially oxidize the cytochrome b of such membranes.(More)
Some evidence for identification of human rosette-forming cells as thymus and thymus-dependent lymphocytes (T cells) is presented. Application of the rosette assay to several clinical conditions including primary immunodeficiency diseases indicates the usefulness of this assay in quantitating the number of T cells in peripheral blood.
Isolated membranes of Bacillus stearothermophilus 2184D can be disrupted by treatment with sodium dodecyl sulfate (SDS). This disruption is attended by a decreased turbidity of membrane suspensions and a differential loss of activities of the electron transport system. Reduced methyl viologen (MVH)-nitrate reductase activity is insensitive to SDS treatment,(More)
An antihuman T-cell has been prepared in rabbits by infecting lymphocytes from a patient with Bruton-type agammaglobulinemia and absorption with cultured B-lymphoblast cells. Cytotoxicity of the antiserum was assayed with peripheral blood lymphocytes in the presence of rabbit complement and its specificity for T vs B cells was verified. This anti-T-cell(More)
It is known that the impairment of cell-mediated immunity (CM) exists in lepromatous leprosy patients. This was shown by 1) delayed skin reaction to various test antigens, 2) decreased transformation of lymphocytes by various antigens, 3) delayed rejection of skin allograft, 4) decreased migration inhibitory fa-tor (MIF) formation, and 5) histologic study(More)