D. F. Hanson

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Endogenous pyrogen (EP) and interleukin (IL 1) appear to be closely related or identical. We therefore conducted experiments to test whether the central action of EP (fever) enhanced its local action (potentiation of immune responses). We found that 3H-TdR incorporation by thymocytes stimulated with IL 1 in the presence of 5 micrograms/ml of(More)
Fever is a common sign of clinical disease but its biological purpose is unclear. Several cytokines mediate fever as well as the regulation of immune responses, and it was previously shown that murine thymocyte proliferative responses to IL-1 and IL-2 are exquisitely temperature-sensitive. The present studies were performed to determine whether such a high(More)
Rabbit mononuclear cells from oil-induced peritoneal exudates were purified by centrifugation on Percoll gradients, suspended in tissue culture medium, and stimulated with opsonized Staphylococcus epidermidis. The supernatants from these macrophages caused fever when injected intravenously into rabbits (endogenous pyrogen [EP] activity). The EP activity was(More)
We biochemically identified 235 Citrobacter strains to the species level on the basis of the recently proposed taxonomic changes of Brenner et al. (D. J. Brenner, P. A. D. Grimont, A. G. Steigerwalt, G. R. Fanning, E. Ageron, and C. F. Riddle, Int. J. Syst. Bacteriol. 43:645-658, 1993). Citrobacter isolates were initially identified as C. koseri or as(More)
Recent studies in our laboratory have shown that IL 1-dependent mitogenic activity is present in apparently homogeneous preparations of rabbit endogenous pyrogen. This finding suggested that the mitogenic and pyrogenic activities of this molecule might serve a common goal. Initial studies on the temperature dependence of interleukin-dependent thymocyte(More)
The purpose of this study was to clarify the association between the oral infectivity of a bacterial strain and its susceptibility to ingestion by mononuclear phagocytes or ability to survive within them. Ten bacterial strains tested--all of known oral infectivity--comprised Salmonella typhimurium, Listeria monocytogenes (three strains), Escherichia coli(More)
Fever's ability to manipulate the character and extent of physiological temperature gradients correlates with the unusual influence different physiological temperatures have upon model immune responses in vitro. This relationship may help to explain the remarkable evolutionary conservation of the febrile response to infection. A very restricted range of the(More)
Cells obtained from acute peritoneal exudates in rabbits were separated into neutrophil and mononuclear populations by centrifugation on colloidal silica gradients. When these populations were separately incubated in tissue culture medium in the presence of opsonized Staphylococcus epidermidis, endogenous pyrogen was secreted only by the adherent cells of(More)
Benign laryngeal lesions were examined for patterns of injury indicated by deposition of fibronectin and collagen type IV. An immunoperoxidase technique was used to compare 33 fresh or paraffin-embedded tissues with regard to their staining of monoclonal antibodies directed against fibronectin and collagen type IV. Two types of patterns were recognized. One(More)