D Cheyvialle

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The gene encoding MSVSP99 (mouse seminal vesicle secretory protein of 99 amino acids) is specifically expressed in the mouse seminal vesicle under androgenic control. To study hormonal regulation, fragments of the 5'-flanking region, extending from -2365 to +16 were linked to the chloramphenicol acetyl transferase (CAT) gene and cotransfected with an(More)
We present a reliable, simple, and quick system for screening antibody-antigen complexes on nitrocellulose. The apparatus necessary for this system is inexpensive and easy to use, and it can be adapted to blot or dot analysis without any modification. The number of antibody-antigen combinations that can be tested in one experiment ranges from 25 to 31 for(More)
Pure epithelial cell cultures, obtained from primary culture of vas deferens tissue collected from 20- to 30-day-old mice, were amplified by subculturing the cells over 3T3 feeder layer in a serum-free defined medium. Adhesion and proliferation of epithelial cells did not require androgens, but a minimal concentration of 5.10(-7) M hydrocortisone. In that(More)
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