D C Deitch

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a Mutated ras genes (Ha-, Ki-and N-ras) have been found in a variety of human tumours (Barbacid, 1987). Although in some tumour types the incidence of mutations is relatively high (Bos, 1989), the practical value of these findings in cancer diagnosis and/or prognosis has not been established. Here we report the detection of a mutation of Ha-ras gene codon(More)
This report concerns the study of Ha-ras gene mutations and ras p21 expression in primary tumors of the urinary bladder. Polymerase chain reaction-based techniques and computerized image analysis were used. The data obtained were related to tumor grade, DNA ploidy, and tumor invasion. A point mutation (G-->T) at Ha-ras codon 12 was found in 30 of 67 tumors.(More)
Comparative DNA ploidy measurements were carried out by flow cytometry and by image analysis on cells in 71 bladder washing specimens from 50 patients with past histories of bladder tumors. Among the specimens classified as diploid or questionable by flow cytometry, 14 showed the presence of aneuploid DNA values documented by image analysis. In 18 of the 50(More)
Two patients suffered minor head injuries diagnosed as cerebral concussions. Both had normal CTs in the emergency room but developed progressive neurologic symptoms weeks later. Both had large subdural hematomas requiring surgical drainage. Physicians should be alert for neurologic deterioration in patients with minor head injuries, even after normal CTs.
SUMMARY One hundred fifty rats (with equal number of controls) were exposed to the inhalation of beryllium sulfate aerosol at a mean atmospheric concentration of 34.25 ¿ig of Be/cu m, and sacrificed in monthly groups during 72 weeks of exposure. Average lung weight towards the end of exposure was 4.25 times normal. Histopathologic examination disclosed 2(More)
The DNA ploidy and cell-cycle distribution of three separate fresh tissue samples of 60 colorectal adenocarcinomas were analyzed by flow cytometry. DNA ploidy was concordant among the three samples in 38 cases (63.3%). In the remaining 22 cases (36.6%), the DNA histograms of two of the three multiple samples were similar; however, the ploidy of the third(More)
The oncogenes most frequently detected in human tumors belong to the ras gene family (Ha-ras, Ki-ras, and N-ras). These genes encode a group of closely related 21,000 dalton proteins termed p21. An immunohistochemical study of ras p21 expression was carried out on paraffin sections of 54 human breast carcinomas using monoclonal antibodies to p21. The(More)
Expression of the c-myc gene is often elevated in human colorectal tumors, but reported amplification of the locus is rare. Here we demonstrate that modest amplification of c-myc is frequently found in aggressive subtypes of colorectal cancer. Careful quantitation of c-myc copy number has shown amplification in 53.8% (7/13) mucinous tumors, 42.3% (3/7)(More)
DNA ploidy and cell cycle phases of benign human colonic epithelium peripheral to adenocarcinoma were analyzed by flow cytometry in 188 prospective cases. Human colonic epithelium was shown to be diploid with a mean DNA index (DI) of 1.01. The G0G1 compartment accounted for nearly 93% of the cells with the remainder in the S and G2+M phases. Parallel(More)
OBJECTIVE The goal of the current study was to directly assess the role of loss of mucosal barrier function in nutritionally induced bacterial translocation. BACKGROUND Parenteral and certain elemental enteral diets have been shown to promote bacterial translocation. The mechanisms underlying this observation, especially the question of whether(More)