D. Bubeníková

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The present study was designed to examine the kinetics and function of peritoneal exudate cells (PEC) during local interleukin 2 (IL-2) gene therapy of the X63-Ag8.653 plasmacytoma growing in the peritoneal cavity. BALB/c mice were inoculated i.p. on day 0 with a tumorigenic dose of the syngeneic plasmacytoma and on day 1 with non-tumorigenic plasmacytoma(More)
To isolate a stable tumor cell line source of IL-2 (TCGF), 19 murine T-cell lines and their derivatives were screened for both constitutive and mitogen-stimulated IL-2 production. The cloned subline of a mouse thymic lymphoma EL-4 designated as EL-4TF could be stimulated with PMA to produce 80 U/ml of IL-2. A TK- EL-4TFR mutant line has been selected from(More)
We have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1. Peritumoral administration of IL-2-producing RAT-1 cells into congenitally athymic (nu/nu) mice carrying(More)
The effects of long-term internal contamination with 13.3 kBq kg-1 239Pu injected intravenously were studied in 10-week-old ICR (SPF) female mice. Radiosensitivity of spleen colony-forming units (CFU-S) and 125IUdR incorporating into proliferating cells of vertebral bone marrow and spleens were determined in plutonium-treated and control animals one year(More)
Female ICR mice were injected intravenously with low activities of 239Pu (3.0 kBq, 6.0 kBq, 12.3 kBq/kg). In these mice with high spontaneous incidence of hemoblastoses the occurrence of myeloid leukemia, lymphocytic leukemia, lymphosarcoma, reticulum-cell sarcomas and osteosarcoma was studied. Hemoblastoses, on the whole, remained in their numbers(More)
Cytologic and histologic evaluation of granulocytic leukemia was carried out in 22 plutonium-treated mice and in 17 controls. The contaminated animals were injected i. v. with 5.4 kBq 239Pu/mouse (180 kBq/kg) at the age of 70 days. Morphologic evidence of the disease was based on the identification of immature abnormal granulocytes in the bone marrow and(More)
Incidence of myeloid leukemia was studied in 79 mice injected i.v. with 180 kBq 239Pu/kg. Plutonium-treated mice were compared with 70 controls of the same origin and sex (random-bred ICR-SPF female mice). The animals were killed moribund and the disease was diagnosed on the basis of histological and cytologic examination of bone marrow, spleen, liver,(More)
Anodic electrophoretic mobility of thymocytes and lymph node cells from random-bred ICR Swiss mice was examined. Three cell populations were identified which differed in their surface charge as reflected in their distinct mean AEM and in their time of appearance during the course of life. The first electrophoretically slow-moving population with a mean AEM(More)
Experiments were designed to investigate a possible therapeutic role of interleukin-2 (IL-2) gene transfer in the model of murine (EL-4) leukaemia pretreated with cyclophosphamide. It has been found that i.p. pretreatment of the leukaemic mice with cyclophosphamide, followed by i.v. administration of irradiated cells, genetically engineered to produce IL-2(More)
Osteosarcomas were induced in mice by three activities of 226Ra (8.8 micronCi, 24.6 micronC and 70.5 micron Ci/kg) and by three activity levels of 224Ra administered in fractionated doses during a period of 75 weeks (total activity per mouse: 0.65 micron Ci, 1.88 micron Ci). In the 226Ra series 74 cases and in the 224Ra series 89 cases of osteosarcomas were(More)