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After the light-induced charge separation in the photosynthetic reaction center (RC) of Rhodobacter sphaeroides, the electron reaches, via the tightly bound ubiquinone Q A , the loosely bound ubiquinone Q B. After two subsequent flashes of light, Q B is reduced to ubiquinol Q B H 2 , with a semiquinone anion Q 3 B formed as an intermediate after the first(More)
Red fluorescent proteins (RFPs) are indispensable tools for deep-tissue imaging, fluorescence resonance energy transfer applications, and super-resolution microscopy. Using time-resolved optical spectroscopy this study investigated photoinduced dynamics of three RFPs, KillerRed, mRFP, and DsRed. In all three RFPs, a new transient absorption intermediate was(More)
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